Candida Albicans
Methods and Protocols
(Sprache: Englisch)
Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of...
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Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiasis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful "Methods in Molecular Biology(TM)" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology.
Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology.
Klappentext zu „Candida Albicans “
Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiasis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful "Methods in Molecular Biology(TM)" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology.
There have been remarkable advances in the study of human pathogenic fungi. In this authoritative book expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular.
Inhaltsverzeichnis zu „Candida Albicans “
- Candida Albicans: Methods and Protocols- Table of Contents
- Preface
- Contributors
- Immunogical methods
1. Isolation of dendritic cells (DC) from human blood for in vitro interaction studies with fungal antigens
- T. Sreevalsan
2. Detection and quantitiaton of anti-fungal SlgA antibodies in body fluids
- Michael F. Cole
3. Phagocytosis and killing assays for Candida species
- Chen Du and Richard Calderone
4. Immunization protocols for use in animal models of candidiasis
- Esther Segal and Hana Sandovsky-Losica
- Virulence and biofilms
5. Penetration of antifungal agents through Candida biofilms
- L. Julia Douglas
6. Candidal bioflm analysis in the artificial throat using FISH
- Bastiaan P. Krom Kevin Buijssen, Henk J Bussceher, and Henny C. van der Mei
7. Conditions for optimal Candida biofilm development in microtiter plates
- Bastiaan P. Krom, Jesse Cohen, Gail McElhaney-Feser, Henk Busscher, Henny C, van der Mei and Ronald Cihlar
- Virulence measurements: In vitro, ex vivo, and in vivo
8. Animal models of candidiasis
- Cornelius J. Clancy, Shaoji Cheng and Minh Hong Nguyen
9. Candida albicans gene expression in an in vivo infection model
- Michael Kruppa
10. In vitro and ex vivo assays of virulence in Candida albicans
- Richard Calderone
- Strain typing and identification
11. Biotyping of Candida albicans and other fungi by yeast killer toxins sensitivity
- Luciano Polonelli and Stefania Conti
12. DNA fingerprinting Candida species
- Claude Pujol and David R. Soll
- Genomics and Proteomics
13. The application of tandem affinity purification to Candida albicans
- Chris Blackwell and Jeremy D. Brown
14. Preparation of samples for proteomic analysis of the Candida albicans cell wall
- Neeraj Chauhan
15. Reporter Gene assays in Candida albicans
- Joy Sturtevant
16. Genetic Transformation of Candida albicans
- Ana M. Ramon and William A.
... mehr
Fonzi
17. Large-scale gene disruption using the UAU1 Cassette
- Clarissa J.Nobile and Aaron P Mitchell
- Appendix:
- Neeraj Chauhan and Michael Kruppa
17. Large-scale gene disruption using the UAU1 Cassette
- Clarissa J.Nobile and Aaron P Mitchell
- Appendix:
- Standard growth media and common techniques for use with Candida albicans
- Neeraj Chauhan and Michael Kruppa
... weniger
Bibliographische Angaben
- 2009, 2009, 206 Seiten, Maße: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben:Cihlar, Ronald L.; Calderone, Richard A.
- Herausgegeben: Richard A. Calderone, Ronald L. Cihlar
- Verlag: Humana Press
- ISBN-10: 1588297608
- ISBN-13: 9781588297600
- Erscheinungsdatum: 05.02.2009
Sprache:
Englisch
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