Cardiovascular Development
Methods and Protocols
(Sprache: Englisch)
sCongenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due to the advancement of...
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Produktinformationen zu „Cardiovascular Development “
sCongenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due to the advancement of modern molecular biology and new available equipments and techniques. In Cardiovascular Development: Methods and Protocols expert researchers in the field in the field detail many of the methods which are now commonly used in the field of cardiovascular development research. These include methods and technique for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cardiovascular Development: Methods and Protocols seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research including in vivo imaging and Bioinformatics.
Authoritative and practical, Cardiovascular Development: Methods and Protocols seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research including in vivo imaging and Bioinformatics.
Klappentext zu „Cardiovascular Development “
sCongenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due to the advancement of modern molecular biology and new available equipments and techniques. In Cardiovascular Development: Methods and Protocols expert researchers in the field in the field detail many of the methods which are now commonly used in the field of cardiovascular development research. These include methods and technique for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiovascular Development: Methods and Protocols seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research including in vivo imaging and Bioinformatics.
Inhaltsverzeichnis zu „Cardiovascular Development “
PrefaceContributors Part I: Model organisms 1. Use of Whole Embryo Culture for Studying Heart Development Calvin T. Hang, and Ching-Pin Chang 2. Quantifying Cardiac Functions in Embryonic and Adult ZebrafishTiffany Hoage, Yonghe Ding, and Xiaolei Xu 3. Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting Ching-Pin Chang 4. Morpholino Injection in Xenopus Panna Tandon, Chris Showell, Kathleen Christine, and Frank L Conlon 5. Chicken Chorioallantoic Membrane Angiogenesis ModelDomenico Ribatti 6. Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography Christopher E. Schmitt, Melinda B. Holland, and Suk-Won Jin 7. Whole-mount Confocal Microscopy for Vascular Branching MorphogenesisYoh-suke Mukouyama, Jennifer James, Joseph Nam, and Yutaka Uchida 8. Visualization of Mouse Embryo Angiogenesis by Fluorescence-based Staining Yang Liu, Marc Antoniyak, and Xu Peng 9. Miniaturized Assays of Angiogenesis in Vitro May J. Reed and Robert B. Vernon Part II: Cell and Molecular Biology Methods 10. Analysis of the Endocardial-to-Mesenchymal Transformation of
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Heart Valve Development by a Collagen Gel Culture Assay Ching-Pin Chang, Yiqin Xiong, and Bin Zhou. 11. Quantification of Myocyte Chemotaxis: A role for FAK in Regulating Directional Motility Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor 12. Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining Yanping Zhang and L. Bruno Ruest 13. Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte Jieli Li, Marc Antonyak, and Xu Peng 14. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng 15. Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells Bin Zhou and William T. Pu 16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization Jan Patterson, Rick Metz, and Emily Wilson 17. C-kit Expression Identifies Cardiac Precursor Cells Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny 18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols Dongfei Qi and Mingui Fu 19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja 20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat PupsHoney B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales, Xu Peng, and David E. Dostal Part III: New Techniques 21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways Shenyuan Zhang and Hongying Zheng 22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor 12. Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining Yanping Zhang and L. Bruno Ruest 13. Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte Jieli Li, Marc Antonyak, and Xu Peng 14. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng 15. Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells Bin Zhou and William T. Pu 16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization Jan Patterson, Rick Metz, and Emily Wilson 17. C-kit Expression Identifies Cardiac Precursor Cells Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny 18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols Dongfei Qi and Mingui Fu 19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja 20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat PupsHoney B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales, Xu Peng, and David E. Dostal Part III: New Techniques 21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways Shenyuan Zhang and Hongying Zheng 22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan Bin Zhou and William T. Pu 16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization Jan Patterson, Rick Metz, and Emily Wilson 17. C-kit Expression Identifies Cardiac Precursor Cells Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny 18. Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols Dongfei Qi and Mingui Fu 19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja 20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat PupsHoney B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales, Xu Peng, and David E. Dostal Part III: New Techniques 21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways Shenyuan Zhang and Hongying Zheng 22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy 23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama Natarajan Honey B. Golden, Suraj Sunder, Xu Peng and David E. Dostal 24. Isolation and Preparation of RNA from Rat Blood and Lymphatic Microvessels for Use in Microarray Analysis Eric A. Bridenbaugh 25. Visual Data Mining of Co-expression Data to Set Research Priorities in Cardiac Development Research Vincent VanBuren 26. High-speed Confocal Imaging of Zebrafish Heart DevelopmentMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama NatarajanMichael P. Craig and Jay R. Hove 27. Measurement of Electrical Conduction Properties of Intact Embryonic Murine Hearts by Extracellular Microelectrode ArraysDavid G. Taylor and Anupama NatarajanDavid G. Taylor and Anupama Natarajan
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Bibliographische Angaben
- 2012, 2012, 356 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Marc Antonyak, Xu Peng
- Verlag: Humana Press
- ISBN-10: 1617795224
- ISBN-13: 9781617795220
- Erscheinungsdatum: 06.01.2012
Sprache:
Englisch
Rezension zu „Cardiovascular Development “
From the reviews:"This is an up-to-date review of current research techniques used in the field of cardiovascular development. ... The book successfully instructs researchers on proper experimental techniques, giving step-by-step instructions, required for research in this field. ... this book is intended for use by both novice and experienced basic science researchers. ... This book is an excellent reference for any researcher in this area." (Patrick T. Campbell, Doody's Book Reviews, April, 2012)
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