Conjugation and Deconjugation of Ubiquitin Family Modifiers

Preface: New Paradigms in the Conjugation of Ubiquitin Family Modifiers xviiMarcus Groettrup1. Activation of Ubiquitin and Ubiquitin?Like Proteins 1Frederick C. Streich, Jr. and Arthur L. Haas2. Control of Ubiquitin Conjugation by Cdc48 and Its Cofactors 17Alexander Buchberger3. Regulation of Ubiquitin Receptors by Coupled Monoubiquitination 31Daniela Hoeller and Ivan Dikic4. Control of Cullin?Ring Ubiquitin Ligase Activity by Nedd8 41Raymond J. Deshaies, Ethan D. Emberley and Anjanabha Saha5. Control of Deneddylation by the Cop9 Signalosome 57Tilo Schmaler and Wolfgang Dubiel6. Mechanism, Specificity and Structure of the Deubiquitinases 69David Komander7. Ubiquitin Conjugation and Deconjugation in NF?kB Signaling 88Michael Düwel, Kamyar Hadian and Daniel Krappmann8. Functions of Linear Ubiquitin Chains in the NF?kB Pathway: Linear Polyubiquitin in NF??B Signaling 100Kazuhiro Iwai9. Assembly of K11?Linked Ubiquitin Chains by the Anaphase?Promoting Complex 107Michael Rape10. Ubiquitin Family Members in the Regulation of the Tumor Suppressor p53 116Dimitris P. Xirodimas and Martin Scheffner11. Ubiquitylation in the ERAD Pathway 136Frederik Eisele, Antje Schäfer and Dieter H. Wolf12. Pupylation: A Signal for Proteasomal Degradation in Mycobacterium tuberculosis 149Kristin E. Burns and K. Heran Darwin13. sumo Control 158Katharina Maderböck and Andrea Pichler14. The In Vivo Functions of Desumoylating Enzymes 170Tasneem Bawa?Khalfe and Edward T.H. Yeh15. Regulatory Functions of Ubiquitin and Sumo in DNA Repair Pathways 184Stefan Jentsch and Stefan Müller16. Sumoylation as a Signal for Polyubiquitylation and Proteasomal Degradation 195Maria Miteva, Kirstin Keusekotten, Kay Hofmann, Gerrit J.K. Praefcke and R. Jürgen Dohmen17. In Vivo Functions of Isgylation 215Klaus?Peter Knobeloch18. Identification and Validation of ISG15 Target Proteins 228Larissa A. Durfee and Jon M. Huibregtse19. FAT 10: Activated by UBA6and Functioning in Protein Degradation 238Christiane Pelzer and

MARCUS GROETTRUP is a Professor for Immunology in the Department ofBiology of Konstanz University in Germany. His main interests are in the role of the immunoproteasome in antigen fragmentation and autoimmunity. Moreover, he has studied the function and conjugation of the ubiquitin-like protein FAT10. He studied biochemistry in Tübingen and ET H Zürich and did his diploma thesis in the laboratory of H. Hengartner and R. Zinkernagel. During his PhD at the Basel Institute for Immunology with H. von Boehmer he discovered the pre T cell receptor. After habilitation at Humboldt University Berlin in the group of P-M. Kloetzel on the topic of antigen processing he founded his own group at the Cantonal Hospital St. Gallen, Switzerland. Since 2002 he holds the Chair of Immunology at the University of Konstanz, Germany. Several prizes were awarded to Dr. Groettrup like the Award of the Sandoz Foundation for Therapeutic Research, the Karl Lohmann Prize of the German Society for Biological Chemistry, the Langener Science Prize of the Paul Ehrlich Institute, and the Research Award by the CaP CURE foundation.