Coupled Bioluminescent Assays
Methods, Evaluations, and Applications
(Sprache: Englisch)
This book highlights the applications of coupled bioluminescence assay techniques to real world problems in drug discovery, environmental and chemical analysis, and biodefense. It separates theoretical aspects from the applied sections in a clear and readable way.
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Produktinformationen zu „Coupled Bioluminescent Assays “
This book highlights the applications of coupled bioluminescence assay techniques to real world problems in drug discovery, environmental and chemical analysis, and biodefense. It separates theoretical aspects from the applied sections in a clear and readable way.
Klappentext zu „Coupled Bioluminescent Assays “
Brings the field of coupled bioluminescence to mainstream scienceThis book enables readers to take full advantage of coupled bioluminescent (CB) assay techniques by demonstrating how they are used to solve real-world problems. It guides readers involved in drug discovery and biomedicine through a broad range of valuable CB techniques, including assays of kinases, phosphatases, and other enzymes; cytotoxicity measurements; and high-throughput screening of receptor modulators. In each case, CB methods are compared with competing technologies to enable informed choices. In addition, the book's coverage of CB methods in biomonitoring; analysis of nitrate, a critical marker of explosives residue and fertilizer runoff; and nerve agent and pesticide detection supports the work of researchers involved in biodefense as well as environmental and food sciences.
Coupled Bioluminescent Assays is divided into three parts:
* Background to coupled bioluminescent assays
* Biomedical applications of coupled bioluminescence
* Other applications of coupled bioluminescence
Highlights of coverage include:
* CB cytotoxicity assays
* CB assays of kinases and phosphatases
* CB measurements of G protein coupled receptor activity
* Assessment of nitric oxide synthase activity by CB
* CB determination of bioburden and sterility
* Emerging regulatory concerns
Each chapter clearly presents both underlying theory and applications. References facilitate further investigations by guiding readers to the primary literature.
CB techniques offer advantages in speed and sensitivity that fluorometric techniques generally cannot match. Bioluminescent reaction schemes are typically one to several orders of magnitude more sensitive than fluorometric assays, yet these techniques have been vastly underutilized. With this book as their guide, chemists, biochemists, and molecular biologists can take full advantage of a broad range of available CB assays to
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achieve their research goals.
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This book highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense. It separates theoretical aspects from the applied sections in a clear and readable way. Coupled Bioluminescent Assays, explains the uses of CB technologies across drug discovery to analyze toxicity, drug receptors, and enzymes. It covers applications in environmental analysis and biodefense, including cytotoxicity, fertilizer and explosives analysis, and nerve agent and pesticide detection. This is the premier reference on coupled bioluminescent assays for chemists, biochemists, and molecular biologists.
Inhaltsverzeichnis zu „Coupled Bioluminescent Assays “
PREFACEPART I BACKGROUND TO COUPLED BIOLUMINESCENT ASSAYS
1 Introduction
1.1 Introduction to Coupled Bioluminescent Assays
1.2 Luminescent Technologies of the Life Sciences
1.3 Varieties of Fluorometric Assays
1.4 Chemiluminescence and Bioluminescence
1.5 Common Bioluminescence Systems
1.6 A Coupled Bioluminescent Reaction
1.7 Summary
2 Coupled Bioluminescent Reactions in Practice
2.1 Principles of Coupled Bioluminescent Reactions
2.2 Instrumentation and Equipment for Coupled Bioluminescent Assays
2.3 Coupled Bioluminescent Assay Procedures and Precautions
2.4 Data Handling for Coupled Bioluminescent Assays
2.5 Comparison of Coupled Bioluminescent Assays with Other Methods
PART II BIOMEDICAL APPLICATIONS OF COUPLED BIOLUMINESCENCE
3 Coupled Bioluminescent Cytotoxicity Assays
3.1 Introduction
3.2 Membrane Integrity Assays
3.3 A Coupled Bioluminescent Assay for Enzyme Release: The G3PDH-Release Assay
3.4 Viability Assays
4 The Role of Coupled Bioluminescent Assays in Kinase Screening and Study
4.1 The Many Roles of Kinases in Biology
4.2 Current Standard Kinase Assays
4.3 Coupled Bioluminescent Kinase Assays
4.4 Conclusions
5 Coupled Bioluminescent Phosphatase Assays
5.1 Introduction
5.2 Phosphatases
5.3 Contemporary Phosphatase Assay Technologies
5.4 CB Phosphatase Assays
5.5 Conclusions
6 Acetylcholinesterase
6.1 Introduction
6.2 Established AChE Assay Methods
6.3 Recent Developments in AChE Assay Methods
6.4 Evaluation of Current AChE High-Throughput Screening Methods
6.5 Coupled Bioluminescent Assays of AChE Activity
6.6 Comparison of Coupled Bioluminescent and Other Methods of Measuring AChE Activity
7 Measurement of Nitric Oxide Synthase Activity by Coupled Bioluminescence
7.1 Introduction
7.2 Current NOS Assays
7.3 Conclusions
8 The Coupled Bioluminescent Pyrophosphorolysis Assay
8.1 Introduction
8.2 Genetic Variation in
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Modern Medicine
8.3 DNA Complementarity
8.4 The READIT(r) Pyrophosphorolysis Assay
9 Coupled Luminescent Assays of G-Protein-Coupled Receptors
9.1 Introduction to G-Protein-Coupled Receptors
9.2 GPCR Assay Methods
9.3 Summary
10 Coupled Bioluminescent Protease Assays
10.1 Introduction
10.2 Protease Assays
10.3 Coupled Bioluminescent Protease Assays
10.4 Summary
11 Coupled Luminescent Assays Involving Aequorin
11.1 Introduction to Aequorin
11.2 Detection of Calcium in Practice
12 Coupled Bioluminescent Reporter Assays
12.1 Introduction to Reporter Assays
12.2 Luciferases as Reporters of Promoter Activities
12.3 Aequorin as a Reporter Enzyme
12.4 Vectors for Use in Reporter Assays
12.5 Summary
13 Coupled Bioluminescent Assays: Regulatory Concerns
13.1 Introduction
13.2 Regulatory Aspects of Assay Development
13.3 Summary
PART III OTHER APPLICATIONS OF COUPLED BIOLUMINESCENCE
14 Coupled Bioluminescent Determination of Bioburden and Sterility
14.1 Introduction
14.2 Rapid Methods of Bioburden and Sterility Assessment
15 Environmental Applications of Coupled Bioluminescent Assays
15.1 Introduction
15.2 Current Methods for Environmental Monitoring of Water Quality
15.3 Methods of Monitoring Stream Water and Lake Water
15.4 Methods of Monitoring Drinking Water
Appendix A: One-Letter Amino Acid Abbreviations
Glossary
Bibliography
Index
8.3 DNA Complementarity
8.4 The READIT(r) Pyrophosphorolysis Assay
9 Coupled Luminescent Assays of G-Protein-Coupled Receptors
9.1 Introduction to G-Protein-Coupled Receptors
9.2 GPCR Assay Methods
9.3 Summary
10 Coupled Bioluminescent Protease Assays
10.1 Introduction
10.2 Protease Assays
10.3 Coupled Bioluminescent Protease Assays
10.4 Summary
11 Coupled Luminescent Assays Involving Aequorin
11.1 Introduction to Aequorin
11.2 Detection of Calcium in Practice
12 Coupled Bioluminescent Reporter Assays
12.1 Introduction to Reporter Assays
12.2 Luciferases as Reporters of Promoter Activities
12.3 Aequorin as a Reporter Enzyme
12.4 Vectors for Use in Reporter Assays
12.5 Summary
13 Coupled Bioluminescent Assays: Regulatory Concerns
13.1 Introduction
13.2 Regulatory Aspects of Assay Development
13.3 Summary
PART III OTHER APPLICATIONS OF COUPLED BIOLUMINESCENCE
14 Coupled Bioluminescent Determination of Bioburden and Sterility
14.1 Introduction
14.2 Rapid Methods of Bioburden and Sterility Assessment
15 Environmental Applications of Coupled Bioluminescent Assays
15.1 Introduction
15.2 Current Methods for Environmental Monitoring of Water Quality
15.3 Methods of Monitoring Stream Water and Lake Water
15.4 Methods of Monitoring Drinking Water
Appendix A: One-Letter Amino Acid Abbreviations
Glossary
Bibliography
Index
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Autoren-Porträt von Michael J Corey
Michael J. Corey, PHD, is a consulting biochemist and a leading authority on coupled bioluminescent assays. A former head of assay development in biotechnology, he is a founding member of the Scientific Advisory Board of Cell Technology, Inc. Dr. Corey holds key patents in the field of coupled bioluminescence.
Bibliographische Angaben
- Autor: Michael J Corey
- 2008, 1. Auflage, 312 Seiten, Maße: 16,2 x 24,3 cm, Gebunden, Englisch
- Verlag: Wiley & Sons
- ISBN-10: 0470108835
- ISBN-13: 9780470108833
- Erscheinungsdatum: 13.01.2009
Sprache:
Englisch
Pressezitat
"The chapters of the book are well balanced ... .Recommended to scientist and students who want to improve their knowledge of bioluminescent coupled assays and bioluminescence in general." ( Analytical and Bioanalytical Chemistry , August 2009)
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