Diagnostic Cytogenetics

Part I Classical Cytogenetics:

01 Tissue Culture, Friedel Wenzel

02 Chromosome staining, Angelika Köhler

03 Karyotyping and Data Interpretation, Karsten Held

04 Documentation, Peter Miny, Rolf-Dieter Wegner

Part II Postnatal Diagnosis:

05 Peripheral Blood, Iris Bartels

06 Establishment of permanent growing lymphoblastoid cell lines, Heidemarie Neitzel

07 Solid tissues, Regine Schubert and Gesa Schwanitz

08 Cells from urine sample, Hannelore Körner, Henrike Dia, Christiane Bommer

09 Classical and molecular cytogenetics of tumor cells, Brigitte Schlegelberger, Simone Metzke, Svetlana Harder, Reina Zühlke-Jenisch,Yanming Zhang, Reiner Siebert

10 Cytogenetics of Meiotic Cells, R. Johannisson

Part III Prenatal Diagnosis:

11 Prenatal Diagnosis - an introduction, Rolf-Dieter Wegner

12 Amniotic fluid cell analysis, Ingo Kennerknecht, Mahmoud Djalali, Gotthold Barbi, Walter Just, Walther Vogel

13 Chorionic villi sampling, Rolf-Dieter Wegner and Holger Toennies

Part IV Special Applications:

14 Diagnosis of chromosomal instability syndromes, Rolf-Dieter Wegner, Markus Stumm

15 Flow Cytometric Testing for Syndromes with Chromosomal Instability and for Aplastic Anemia and Related Hematological Disorders, Detlev Schindler and Holger Hoehn

16 Cell fusion, Markus Stumm and Rolf-Dieter Wegner

17 Origin of Trisomies, Gesa Schwanitz and Thomas Eggermann

Part V Molecular Cytogenetics:

18 Fluorescence in situ hybridization, Gesa Schwanitz and Regine Schubert

19 Fluorescence in situ Hybridization (FISH) Analysis in Human Sperm Cells, Evelyn Ko and Rene Martin

20 Microdissection and Reverse Chromosome Painting, G. Senger, I. Chudoba, J. Weimer and U. Claussen

21 Comparative genomic hybridization (CGH), Traudl Henn, Oskar A. Haas

Part VI Techniques in Development:

22 Fetal cells in maternal blood, Dorothee Gänshirt, Henk S.P.Garritsen, Wolfgang Holzgreve

23 Spectral Karyotyping in Clinical and Tumor