Endocannabinoid Signaling

Need for Methods to InvestigateEndocannabinoid Signaling.- Extraction and Simultaneous Quantificationof Endocannabinoids and Endocannabinoid-Like Lipidsin Biological Tissues.- Determination of 2-Arachidonoylglycerol by µSPE-LC-MS/MS.- Analysis of Omega-3 Fatty AcidDerived N-acylethanolamines in BiologicalMatrices.- Assay of CB1 Receptor Binding.- The Displacement Binding Assay Using HumanCannabinoid CB2 Receptor-Transfected Cells.- Assay of TRPV1 Receptor Signaling.- A Functional Assay for GPR55: Envision Protocols.- The Cyclic AMP Assay Using Human CannabinoidCB2 Receptor-Transfected Cells.- Assay of GTPGammaS Binding in Autoradiography.- Protocol to Study beta-Arrestin Recruitment by CB1 and CB2Cannabinoid Receptors.- Assay of NAT Activity.- Assay of NAPE-PLD Activity.- Assay of FAAH Activity.- Assay of NAAA Activity.- Assay of DAGL /beta Activity.- Assay of Monoacylglycerol Lipase Activity.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD6.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD12.-  Assay of Endocannabinoid Uptake.- Assay of Endocannabinoid Oxidation by Cyclooxygenase-2.- Oxygenation of Anandamide by Lipoxygenases.- Assay of Endocannabinoid Oxidation by Cytochrome P450.-  Assessing Gene Expression of the Endocannabinoid System.- Western Blotting of the Endocannabinoid System.- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Traffickingin Cultured Cells.- Measuring ECS Interaction with Biomembranes.- Visualization of Endocannabinoids in the Cell.