Environmental Microbiology

Methods for Isolation and Cultivation of Filamentous Fungi.- Rapid Extraction of PCR-Competent DNA from Recalcitrant Environmental Samples.- Quantitative PCR for Detection of mRNA and gDNA in Environmental Isolates.- Analysis of Community Dynamics in Environmental Samples Using Denaturing Gradient Gel Electrophoresis.- Terminal Restriction Fragment Length Polymorphism (T-RFLP) Profiling of Bacterial 16S rRNA Genes.- Profiling the Diversity of Microbial Communities with Single-Strand Conformation Polymorphism (SSCP).- Human Fecal Source Identification with Real-Time Quantitative PCR.- Next Generation Barcode Tagged Sequencing for Monitoring Microbial Community Dynamics.- Analysis of Methanotroph Community Structure Using a pmoA-Based Microarray.- Biolog Phenotype MicroArrays for Phenotypic Characterization of Microbial Cells.- Visualization of Metabolic Properties of Bacterial Cells Using Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS).- Single-Cell Raman Sorting.- Bacterial Whole Cell Biosensors for the Detection of Contaminants in Water and Soils.- Stable Isotope Probing to Study Functional Components of Complex Microbial Ecosystems.- Metagenomics Using Next-Generation Sequencing.- Targeted Genomics of Flow Cytometrically Sorted Cultured and Uncultured Microbial Groups.- Quantitative Microbial Metatranscriptomics.- Quantitative Metaproteomics: Functional Insights into Microbial Communities.