Enzyme Immunoassays
From Concept to Product Development
(Sprache: Englisch)
Since the classical work of Rosalyn Yalow and Solomon Berson on the radioim munoassay of insulin in the late 1950s, which so brilliantly opened new domains in the diagnostics sector, immunoassays have been well received as a diagnostic and research tool in...
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Klappentext zu „Enzyme Immunoassays “
Since the classical work of Rosalyn Yalow and Solomon Berson on the radioim munoassay of insulin in the late 1950s, which so brilliantly opened new domains in the diagnostics sector, immunoassays have been well received as a diagnostic and research tool in several disciplines of life sciences. The economics, rapidity, sensitivity, specificity, and the easy-to-use approach of immunodiagnostic tests are widely recognized, and have had a tremendous impact on clinical, agricultural, food, veterinary, and environmental diagnostics. At present, in the clinical diag nostic sector alone, immunoassay products command an annual worldwide mar ket value in excess of U. S. $10 billion, which is expanding by about 10% per annum. The recent trend toward increasing demand for automation of immunoassays and stable reagents clearly shows a definitive shift to nonisotopic immunoassay systems on a large scale. Historically, enzyme immunoassays have been in a prime position for in-house applications,primarily because of the easy access to inex pensive measuring equipment as well as to a vast body of literature on labeling techniques and applications. The recent popularity with the general public of sev eral over-the-counter immunoassay products based on this technology, such as glucose level kits for diabetics, cholesterol screening tests, and pregnancy detec tion test kits, is quite evident.
Inhaltsverzeichnis zu „Enzyme Immunoassays “
Part 1: Basic considerations (concepts): Introduction. Antibody: biochemistry, structure, and function. Antigen: antibody reactions. Conjugation techniques. Antibody production. Enzymes and signal amplification systems. Separation and solid phase systems. Part 2: Product development: Immunoassay classification and commercial technologies. Assay development, evaluation, and validation. Reagent formulations and shelf life evaluation. Data analysis. Documentation, Registration, and Diagnostic Start-Ups.
Bibliographische Angaben
- Autor: S. S. Deshpande
- 1996, 464 Seiten, Maße: 16 x 24,1 cm, Gebunden, Englisch
- Verlag: Springer
- ISBN-10: 0412056011
- ISBN-13: 9780412056017
- Erscheinungsdatum: 30.06.1996
Sprache:
Englisch
Rezension zu „Enzyme Immunoassays “
`... all those who read this book will be far richer in their knowledge of enzyme immunoassays and their application.' Chemistry and Industry `...if you're looking for a book that describes immunoassay methods in general then this one has something to recommend it...if you are looking to go beyond the laboratory and develop commercial assays then this book is worth buying for its second half alone.' Analyst
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