Exocytosis and Endocytosis

Contents Preface Contributors Part I An Overview of Methods for Analyzing Vesicular Trafficking 1 Vesicular Trafficking: Molecular Tools and Targets Elena Vassilieva and Asma Nusrat 2 Pharmacological Inhibition of Endocytic Pathways. Is It Specific Enough to be Useful? Andrei Ivanov Part II Cell-free and Biochemical Assays of Exocytosis and Endocytosis 3 In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion Susanne Kreye, Jörg Malsam, and Thomas H. Söllner 4 Imaging Ca2+-Triggered Exocytosis of Single Secretory Granules on Plasma Membrane Lawns from Neuroendocrine Cells. Thorsten Lang 5 FRAP Analysis of Secretory Granule Lipids and Proteins in the Sea Urchin Egg Julian L. Wong and Gary M. Wessel 6 A Cell-Free Assay for Endocytosis of E-cadherin Toshiaki Sakisaka and Yoshimi Takai 7 Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin Noriyuki Nishimura and Takuya Sasaki 8 Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-epithelial Cells by OptiPrepTM Density Gradient Ultracentrifugation Xuhang Li and Mark Donowitz 9 Identification and Characterization of Interacting Partners of Rab GTPases by Yeast Two-Hybrid Analyses Mark Kail and Angelika Barnekow Part III Analysis of Endocytosis and Exocytosis in Different Cell Systems 10 High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins Roland Govers, David E. James, and Adelle C. F. Coster 11 Studying Phagocytosis by Live-Cell Scintillation Proximity Assay Walter Stockinger and Axel Nohturfft 12 Transcytosis of Polymeric Immunoglobulin A in Polarized Madin-Darby Canine Kidney Cells Asli Oztan, Christine Rondanino, and Gerard Apodaca 13 The Use of SyntaxinChimeras to Study Polarized Protein Trafficking in Epithelial Cells Martin B.A. ter Beest 14 Targeting the Epithelial SNARE Machinery by Bacterial Neurotoxins Veronique Proux-Gillardeaux and Thierry Galli 15 Exocytosis of Endothelial Cells is Regulated by N-ethylmaleimide Sensitive Factor Munekazu