Fertilization and Embryonic Development In Vitro

Prologue.- 1 Collection of Gametes in Laboratory Animals and Preparation of Sperm for in Vitro Fertilization.- Collection of Ova for in Vitro Fertilization.- Methods Presently Used for Sperm Collection.- Differences in Sperm as a Function of Collection Method.- Preparation of Ejaculated Sperm for Use in in Vitro Fertilization.- Methods Used to Induce Capacitation of Ejaculated Sperm.- References.- 2 Ovum Collection and Induced Luteal Dysfunction in Primates.- Ovum Collection.- Evaluation of Corpus Luteum function.- Induced Folliculogenesis.- Discussion and Conclusions.- References.- 3 Analysis of Culture Media for in Vitro Fertilization and Criteria for Success.- Culture Media.- Criteria for Success.- Conclusions.- References.- 4 In Vitro Culture of the Zygote and Embryo.- General Observations on Fertilization and Early Development in Vitro.- Metabolic Requirements for Embryonic Development in Vitro.- Complex Media and Biological Fluids for Embryo Culture.- Conclusions.- References.- 5 Mechanisms of Fertilization in Mammals.- Epididymal Maturation and Capacitation of Spermatozoa.- Acrosome and Acrosome Reaction.- Hyperactivation of Spermatozoa.- Interaction of Spermatozoa with the Cumulus Oophorus.- Interaction of Spermatozoa with the Zona Pellucida.- Sperm-Egg Fusion.- Decondensation of the Sperm Nucleus and Development of Sperm and Egg Pronuclei.- Conclusions.- References.- 6 The Mammalian Egg's Block to Polyspermy.- Zona Reaction.- Zona Reaction Mechanisms.- Egg Plasma Membrane Block.- Block to Polyspermy Mechanisms.- Conclusions.- References.- 7 Gamete Interaction in the Sea Urchin A Model for Understanding the Molecular Details of Animal Fertilization.- Fertilization in the Sea Urchin.- The Plasma Membrane of Sea Urchin Sperm.- Sperm-Specific Surface Antigenicity Common to Seven Animal Phyla.- Isolation of Acrosome Granules and Identification of Bindin as the Major Component Involved in Sperm Adhesion.- Identification of a Bindin Receptor Glycoprotein from the