Functional Proteomics
Methods and Protocols
(Sprache: Englisch)
As the emerging field of proteomics continues to expand at an extremely rapid rate, the relative quantification of proteins, targeted by their function, becomes its greatest challenge. Complex analytical strategies have been designed that allow comparative...
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As the emerging field of proteomics continues to expand at an extremely rapid rate, the relative quantification of proteins, targeted by their function, becomes its greatest challenge. Complex analytical strategies have been designed that allow comparative analysis of large proteomes, as well as in depth detection of the core proteome or the interaction network of a given protein of interest. In Functional Proteomics: Methods and Protocols, expert researchers describe the latest protocols being developed to address the problems encountered in high-throughput proteomics projects, with emphasis on the factors governing the technical choices for given applications. The case studies within the volume focus on the following three crucial aspects of the experimental design: 1) the strategy used for the selection, purification and preparation of the sample to be analyzed by mass spectrometry, 2) the type of mass spectrometer used and the type of data to be obtained from it, and 3) the method used for the interpretation of the mass spectrometry data and the search engine used for the identification of the proteins in the different types of sequence data banks available. As a part of the highly successful Methods in Molecular Biology(TM) series, the chapters compile step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Functional Proteomics: Methods and Protocols is an ideal resource for all scientists pursuing this developing field and its multitudinous data.
Comprehensive and cutting-edge, Functional Proteomics: Methods and Protocols is an ideal resource for all scientists pursuing this developing field and its multitudinous data.
Klappentext zu „Functional Proteomics “
As the emerging field of proteomics continues to expand at an extremely rapid rate, the relative quantification of proteins, targeted by their function, becomes its greatest challenge. Complex analytical strategies have been designed that allow comparative analysis of large proteomes, as well as in depth detection of the core proteome or the interaction network of a given protein of interest. In Functional Proteomics: Methods and Protocols, expert researchers describe the latest protocols being developed to address the problems encountered in high-throughput proteomics projects, with emphasis on the factors governing the technical choices for given applications. The case studies within the volume focus on the following three crucial aspects of the experimental design: 1) the strategy used for the selection, purification and preparation of the sample to be analyzed by mass spectrometry, 2) the type of mass spectrometer used and the type of data to be obtained from it, and 3) the method used for the interpretation of the mass spectrometry data and the search engine used for the identification of the proteins in the different types of sequence data banks available. As a part of the highly successful Methods in Molecular Biology(TM) series, the chapters compile step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Functional Proteomics: Methods and Protocols is an ideal resource for all scientists pursuing this developing field and its multitudinous data.
Inhaltsverzeichnis zu „Functional Proteomics “
PrefaceContributors Part I: Introduction1. A Brief Summary of the Different Types of Mass Spectrometers Used in Proteomics Christine Schaeffer-Reiss2. Experimental Setups and Considerations to Study Microbial Interactions Petter Melin Part II: Proteomics3. Plant ProteomicsEric Sarnighausen and Ralf Reski4. Methods for Human CD8+ T Lymphocyte Proteome Analysis Lynne Thadikkaran, Nathalie Rufer, Corinne Benay, David Crettaz, and Jean-Daniel Tissot5. Label-Free Proteomics of SerumNatalia Govorukhina, Peter Horvatovich, and Rainer Bischoff6. Flow Cytometric Analysis of Cell Membrane Microparticles Monique P. Gelderman and Jan Simak Part III: Protein Expression Profiling7. ExosomesJoost P. J. J. Hegmans, Peter J. Gerber, and Bart N. Lambrecht8. Toward a Full Characterization of the Human 20S Proteasome Subunits and Their Isoforms by a Combination of Proteomic Approaches Sandrine Uttenweiler-Joseph, Stéphane Claverol, Loïk Sylvius, Marie-Pierre Bousquet-Dubouch, Odile Burlet-Schiltz, and Bernard Monsarrat9. Free-Flow Electrophoresis of the Human Urinary Proteome Mikkel Nissum and Robert Wildgruber10. Versatile Screening for Binary Protein-Protein Interactions by Yeast Two-Hybrid Mating Stef J. F. Letteboer and Ronald Roepman11. Native Fractionation: Isolation of Native Membrane-Bound Protein Complexes from Porcine Rod Outer Segments Using Isopycnic Density Gradient Centrifugation Magdalena Swiatek-de Lange, Bernd Müller, and Marius Ueffing12. Mapping of Signaling Pathways by Functional Interaction ProteomicsAlex von Kriegsheim, Christian Preisinger, and Walter Kolch13. Selection of Recombinant Antibodies by Eukaryotic Ribosome Display Mingyue He and Michael J. Taussig14. Production of Protein Arrays by Cell-Free SystemsMingyue He and Michael J. Taussig15. Nondenaturing Mass Spectrometry to Study Noncovalent Protein/Protein and Protein/Ligand Complexes: Technical Aspects and Application to the Determination of Binding StoichiometriesSarah Sanglier, C¿edric Atmanene,
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Guillaume Chevreux, and Alain Van Dorsselaer16. Protein Processing Characterized by a Gel-Free Proteomics ApproachPetra Van Damme, Francis Impens, Joël Vandekerckhove, and Kris Gevaert17. Identification and Characterization of N-Glycosylated Proteins Using Proteomics David S. Selby, Martin R. Larsen, Cosima Damiana Calvano, and Ole Nørregaard Jensen Part IV: Protein Analysis18. Data Standards and Controlled Vocabularies for Proteomics Lennart Martens, Luisa Montecchi Palazzi, and Henning Hermjakob19. The PRIDE Proteomics Identifications Database: Data Submission, Query, and Dataset ComparisonPhilip Jones and Richard Côté20. Searching the Protein Interaction Space through the MINT Database Andreas Zanzoni, Andrew Chatr-aryamontri, Arnaud Ceol, and Gianni Cesareni21. PepSeeker: Mining Information from Proteomic Data Jennifer A. Siepen, Julian N. Selley, and Simon J. Hubbard22. Toward High-Throughput and Reliable Peptide Identification via MS/MS SpectraJian Liu23. MassSorter: Peptide Mass Fingerprinting Data Analysis Ingvar Eidhammer, Harald Barsnes, and Svein-Ole Mikalsen24. Database Similarity Searches Frédéric Plewniak25. Protein Multiple Sequence Alignment Chuong B. Do and Kazutaka Katoh26. Discovering Biomedical Knowledge from the Literature Jasmin Saric, Henriette Engelken, and Uwe Reyle27. Protein Subcellular Localization Prediction Using Artificial Intelligence Technology Rajesh Nair and Burkhard Rost28. Protein Functional Annotation by Homology Raja Mazumder, Sona Vasudevan, and Anastasia N. Nikolskaya29. Designability and Disease Philip Wong and Dmitrij Frishman30. Prism: Protein-Protein Interaction Prediction by Structural Matching Ozlem Keskin, Ruth Nussinov, and Attila Gursoy31. Prediction of Protein Interaction Based on Similarity of Phylogenetic TreesFlorencio Pazos, David Juan, Jose M. G. Izarzugaza, Eduardo Leon, and Alfonso Valencia32. Large Multiprotein Structure Modeling and Simulation: The Need for Mesoscopic ModelsAntoine Coulon, Guillaume Beslon, and Olivier Gandrillon33. Dynamic Pathway Modeling of Signal Transduction Networks: A Domain-Oriented ApproachHolger Conzelmann and Ernst-Dieter Gilles
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Bibliographische Angaben
- 2008, 596 Seiten, mit farbigen Abbildungen, Maße: 15,7 x 23,6 cm, Gebunden, Englisch
- Herausgegeben:Thompson, Julie D.; Schaeffer-Reiss, Christine; Ueffing, Marius
- Herausgegeben: Julie D. Thompson, Christine Schaeffer-Reiss, Marius Ueffing
- Verlag: Humana Press
- ISBN-10: 1588299716
- ISBN-13: 9781588299710
Sprache:
Englisch
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