Fundamentals of Fluorescence Microscopy

Preface.- Acknowledgements.- Dedication.- Ray Optics, Wave Optics and Imaging System Designing.- Basics of Electromagnetic Theory for Fluorescence Microscopy.- Electric Field Effects in Optical Microscopy Systems.- Quantum Description of Radiation Field and Optical Microscopy.- Molecular Physics of Fluorescence Markers.- Basics of Fluorescence and Photophysics.- General Fluorescence Imaging Techniques.- Multiphoton Fluorescence Microscopy.- Super Resolution Fluorescence Microscopy.- Image Reconstruction Methodologies for Fluorescence Microscopy.- Future Prospective of Fluorescence Microscopy.- Appendix I.- Appendix II.- Appendix III.

Alberto Diaspro is Professor of Physics, Biophysics and Advanced Microscopy Methods at the University of Genoa in the Applied Physics Area, IIT Director and PI, and member of the Medical Nanotechnology PhD Advisory Committee (University of Milan).

Further, he heads a research group at IFOM ? FIRC Oncology Institute, Milan, has scientific collaborations with the Institute of Biophysics of the National Research Council (CNR), and founded LAMBS in 2003 (Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy, www.lambs.it).

He has been President of EBSA (European Biophysical Societies' Association, www.ebsa.org) (2009?2011), President of OWLS (Optics with Life Sciences) (2010?2012) and member of the International Relations Committee of the Biophysical Society, www.biophyscs.org. Also, he is active member of the Biophysical Society, EBSA, SPIE, OSA, IEEE, OWLS (Optics Within Life Sciences of the International Commission for Optics, ICO), FOM (Focus on Microscopy) and SIF (Italian Physical Society, www.sif.it). > 200 ISI publications.

Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing) , Academic Editor to the AIP Advances journal and an editorial board member to 'Microscopy, Research and Technique (Wiley).