Gastrointestinal Physiology and Diseases

CRISPR/Cas9 mediated Genome-editing of Mouse Small Intestinal Organoids.- Lentivirus-based Stable Gene Delivery into Intestinal Organoids.- Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells.- An Air-Liquid Interface Culture System for 3D Organoid Culture of Diverse Primary Gastrointestinal Tissues.- Organotypical Tissue Cultures from Fetal and Neonatal Murine Colon.- Using Chamber Technique to Measure Intestinal Epithelial Permeability.- HPLC-based Metabolomic Analysis of Normal and Inflamed Gut.- NMR-based Metabolomic Analysis of Normal and Inflamed Gut.- Analysis of microRNA Levels in Intestinal Epithelial Cells.- Detecting Reactive Oxygen Species Generation and Stem Cell Proliferation in the Drosophila Intestine.- Imaging Inflammatory Hypoxia in the Murine Gut.- Label-free Imaging of Eosinophilic Esophagitis Mouse Models Using Optical Coherence Tomography.- Near Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colon.- Visualization of Signaling Molecules during Neutrophil Recruitment in Transgenic Mice Expressing FRET Biosensors.- In Vivo Myeloperoxidase Imaging and Flow Cytometry Analysis of Intestinal Myeloid Cells.- Macrophage Isolation from the Mouse Small and Large Intestine.- Isolation and Functional Analysis of Lamina Propria Dendritic Cells from the Mouse Small Intestine.- Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells.- Immunotherapy with iTreg and nTreg Cells in a Murine Model of Inflammatory Bowel Disease.- Isolation of Eosinophils from the Lamina Propria of the Murine Small Intestine.- Investigation of Host and Pathogen Contributions to Infectious Colitis Using the Citrobacter rodentium Mouse Model of Infection.- Murine Trinitrobenzoic Acid-induced Colitis as a Model of Crohn's Disease.- Oxazolone-induced Colitis as a Model of Th2 Immune Responses in the Intestinal Mucosa.- The Mongolian Gerbil: A Robust Model of Helicobacter pylori-induced Gastric Inflammation and Cancer.- A