Genome Transcriptome and Proteome Analysis

Introduction.
 
1 General introduction.
 
1.1 Revision of molecular genetics.
 
1.2 The tools of molecular biology.
 
1.3 Specifics of the genome programmes.
 
1.4 The species analysed.
 
2 Linkage maps.
 
2.1. Tools and methods in genetic mapping.
 
2.2 The development of genetic maps.
 
2.3 Radiation hybrid maps.
 
2.4 Conclusion.
 
3 Physical maps.
 
3.1 Local maps or small genomes.
 
3.2 Strategies for physical mapping of the human genome.
 
3.3 Maps of the human genome.
 
3.4 Conclusion.
 
4 Genome sequencing .
 
4.1 Strategic choices.
 
4.2 Prokaryotic genomes.
 
4.3 Genomes of model eukaryotes.
 
4.4 The human genome.
 
4.5 Conclusion.
 
5 Sequencing cDNA and the transcriptome.
 
5.1 Strategies of cDNA sequencing.
 
5.2 The economic stakes.
 
5.3 The analysis of cDNA sequences.
 
5.4 The transcriptome.
 
5.5 Conclusion.
 
6 The proteome.
 
6.1 Basic techniques.
 
6.2 Transgenesis .
 
6.3 Mutagenesis.
 
6.4 Two-dimensional electrophoresis and identification of proteins.
 
6.5 Identification of protein interactions by twin hybrids.
 
6.6 Chip technology.
 
6.7 Analysis of three-dimensional structure.
 
6.8 Conclusions.
 
7 Identification of genes responsible for disease.
 
7.1 Genetic diseases.
 
7.2 Functional cloning and chromosomal anomalies.
 

7.3 Strategy for positional cloning.
 
7.4 What is the future for the cloning of disease-causing genes?
 
7.5 Conclusion.
 
General conclusion.
 
Bibliography.