Hepatitis C
Methods and Protocols
(Sprache: Englisch)
With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative...
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Produktinformationen zu „Hepatitis C “
With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Fully updated and comprehensive, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease.
Fully updated and comprehensive, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease.
Klappentext zu „Hepatitis C “
When I was ?rst approached by Dr. Hengli Tang about writing the foreword to this book, the second edition of Hepatitis C Virus, I was a little puzzled: Book forewords are penned by persons far more famous than I am. Surely there were better people to write this, with a handful of the true visionaries in the ?eld immediately coming to my mind. However, after some thought and self-re?ection, I realized that I might just be the right person for the job. I remember the publication of the ?rst edition of Hepatitis C Virus in 1998, the year I began reading about hepatitis C virus with great interest. I purchased a copy of the ?rst edition from my meager graduate student stipend and read it from cover to cover, along with a variety of hepatitis C publications, to try to get a feel if this hepatitis C virus ?eld was something I might want to work on in the future. At the time, the ?rst edition was among the most comprehensive collection of hepatitis C protocols and methods available in one volume. I was amazed at the progress that had been made in hepatitis C research, and even more amazed as I learned more about the experimental challenges facing the ?eld at that time.
Inhaltsverzeichnis zu „Hepatitis C “
PART I: DETECTION AND QUANTIFICATION OF HCV1. Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays; Hélène Le Guillou-Guillemette and Françoise Lunel-Fabiani
2. Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen; Filippo Ansaldi and Giancarlo Icardi
3. Immunohistochemical Detection of HCV Proteins in Liver Tissue; Oivier Galy, Isabelle Vincent, Philippe Chevallier, Nicole Lyandrat, and Isabelle Chemin
PART II: HCV NONMENCLATURE, GENOTYPING, AND QUASISPECIES
4. Nomenclature and Numbering of the Hepatitis C Virus; Carla Kuiken and Peter Simmonds
5. A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates; Kathryn J. Rolfe, Tim G. Wreghitt, Graeme J.M. Alexander, and Martin D. Curran
6. Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR1) by In Vitro Translation and Mass Spectrometry; Carmen Yea, Melissa Ayers, and Raymond Tellier
PART III: HCV REPLICATION IN VITRO
7. Purification and Crystallization of NS5A Domain I of Hepatitis C Virus; Joseph Marcotrigiano and Timothy L. Tellinghuisen
8. NS5A Phosphorylation and Hyperphosphorylation; Petra Neddermann
9. Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies; Stefania Di Marco, Edwin Rydberg, Matthew Bottomley, and Andrea Carfi
10. Structural and Functional Analysis of the HCV p7 Protein; Nathalie Saint, Roland Montserret, Christophe Chipot, and François Penin
11. HCV Replicons: Overview and Basic Protocols; Volker Lohmann
12. Reverse Transcription PCR Based Sequence Analysis of Hepatitis C Virus Replicon RNA; Timothy L. Tellinghuisen and Brett D. Lindenbach
13. Studying HCV RNA Synthesis In Vitro with Replication Complexes; Wengang Yang and Mingjun Huang
14. Proteomics Study of the Hepatitis C Virus Replication Complexes; Kyungsoo Chang, Tianyi Wang,
... mehr
and Guangxiang Luo
15. Investigation of the Hepatitis C Virus Replication Complex; Volker Brass, Rainer Gosert, and Darius Moradpour
16. Regulation of Interferon Regulatory Factor-3-dependent Innate Immunity by the HCV NS3/4A Protease; Kui Li
17. Selection and Characterization of Drug Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay; Jason M. Robotham, Heather B. Nelson, and Hengli Tang
18. High Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System; Weidong Hao and Rohit Duggal
19. Inhibition of HCV Replication by Small Interfering RNA; Ratna B. Ray and Tastuo Kanda
PART IV: HCV ENTRY AND INFECTION
20. Cellular Receptors and HCV Entry; Mike Flint and Donna M. Tscherne
21. Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp); Birke Bartosch and Francois-Loic Cosset
22. Screening of Small-molecule Compounds as Inhibitors of HCV Entry; Jian-Ping Yang, Demin Zhou and Flossie Wong-Staal
23. Isolation of the JFH-1 Strain and Development of an HCV Infection System; Takaji Wakita
24. Measuring HCV Infectivity Produced in Cell Culture and In Vivo; Brett D. Lindenbach
25. Genotype 1a HCV (H77S) Infection System; MinKyung Yi and Stanley M. Lemon
26. Full Length Infectious HCV Chimeras; Thomas Pietschmann
27. Adaptation of the Hepatitis C Virus to Cell Culture; Artur Kaul, Ilka Wörz and Ralf Bartenschlager
28. Primary Human Hepatocyte Culture for HCV Study; Haizhen Zhu, John Elyar, Robin Foss, Alan Hemming, Eric Hall, Edward L. LeCluyse, and Chen Liu
29. In Vivo Study of HCV in Mice with Chimeric Human Livers; Norman M. Kneteman and Christian Toso
PART V: IMMUNITY AND MODELING
30. Determination of HCV-Specific T Cell Activity; Joo Chun Yoon and Barbara Rehermann
31. Analysis of HCV-Specific T Cells By Flow Cytometry; Masaaki Shiina and Barbara Rehermann
32. Detection of Neutralizing Antibodies Using HCV Pseudo-Particles (HCVpp); Marlène Dreux and François-Loïc Cosset
33. Mathematical Modeling of HCV Infection and Treatment; Harel Dahari, Emi Shudo, Ruy M. Ribeiro, and Alan S. Perelson
15. Investigation of the Hepatitis C Virus Replication Complex; Volker Brass, Rainer Gosert, and Darius Moradpour
16. Regulation of Interferon Regulatory Factor-3-dependent Innate Immunity by the HCV NS3/4A Protease; Kui Li
17. Selection and Characterization of Drug Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay; Jason M. Robotham, Heather B. Nelson, and Hengli Tang
18. High Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System; Weidong Hao and Rohit Duggal
19. Inhibition of HCV Replication by Small Interfering RNA; Ratna B. Ray and Tastuo Kanda
PART IV: HCV ENTRY AND INFECTION
20. Cellular Receptors and HCV Entry; Mike Flint and Donna M. Tscherne
21. Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp); Birke Bartosch and Francois-Loic Cosset
22. Screening of Small-molecule Compounds as Inhibitors of HCV Entry; Jian-Ping Yang, Demin Zhou and Flossie Wong-Staal
23. Isolation of the JFH-1 Strain and Development of an HCV Infection System; Takaji Wakita
24. Measuring HCV Infectivity Produced in Cell Culture and In Vivo; Brett D. Lindenbach
25. Genotype 1a HCV (H77S) Infection System; MinKyung Yi and Stanley M. Lemon
26. Full Length Infectious HCV Chimeras; Thomas Pietschmann
27. Adaptation of the Hepatitis C Virus to Cell Culture; Artur Kaul, Ilka Wörz and Ralf Bartenschlager
28. Primary Human Hepatocyte Culture for HCV Study; Haizhen Zhu, John Elyar, Robin Foss, Alan Hemming, Eric Hall, Edward L. LeCluyse, and Chen Liu
29. In Vivo Study of HCV in Mice with Chimeric Human Livers; Norman M. Kneteman and Christian Toso
PART V: IMMUNITY AND MODELING
30. Determination of HCV-Specific T Cell Activity; Joo Chun Yoon and Barbara Rehermann
31. Analysis of HCV-Specific T Cells By Flow Cytometry; Masaaki Shiina and Barbara Rehermann
32. Detection of Neutralizing Antibodies Using HCV Pseudo-Particles (HCVpp); Marlène Dreux and François-Loïc Cosset
33. Mathematical Modeling of HCV Infection and Treatment; Harel Dahari, Emi Shudo, Ruy M. Ribeiro, and Alan S. Perelson
... weniger
Bibliographische Angaben
- 2nd ed. 2009, 484 Seiten, Maße: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben: Hengli Tang
- Verlag: Humana Press
- ISBN-10: 1588299708
- ISBN-13: 9781588299703
- Erscheinungsdatum: 10.12.2008
Sprache:
Englisch
Rezension zu „Hepatitis C “
From the reviews of the second edition:"This second edition contains much that was not available for the first edition; replicons, tissue culture infection systems, infectious pseudotype particles, etc. ... The chapters are authored by acknowledged experts and are authoritatively written. ... Of particular value are the notes sections which highlight practical pitfalls and how to avoid them. Given the size of the hepatitis C community ... this book will be a success and should adorn the shelves of many laboratories." (David J. Rowlands, Microbiology Today, July, 2009)
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