Histochemistry

Contents
Chapter 1 Introduction
1.1 The contents and theories in Histochemistry
1.2 The basic requirements of Histochemistry methods

Chapter 2 The Tissue Preparation
2.1 Tissue collection
2.2 Fixation
2.3 Tissue washing-up, dehydration and clearance
2.4 Embedding
2.5 Sectioning
2.6 Adherence and mounting
2.7 Buffer

Chapter 3 The Carbohydrate and its Derivatives Histochemistry
3.1 Classification
3.2 Histochemistry methods

Chapter 4 The Nucleic Acid Histochemistry
4.1 DNA histochemical demonstration
4.2 Comparison demonstration of DNA and RNA Histochmistry

Chapter 5 The Lipid Histochemistry
5.1 Overview
5.2 Demonstration of lipids with physical methods
5.3 Demonstration of lipids with chemical methods

Chapter 6 Enzyme Histochemistry
6.1 Enzyme and its basic histochemical theory
6.2 Histochemistry for common enzymes

Chapter 7 The Basic Theory of Immunohistochemistry
7.1 Basic Immunology
7.2 Common markers and their detection
7.3 Basic conditions

Chapter 8 Common Methods Used in Immunohistochemistry
8.1 Principles
8.2 Immunofluorescence method
8.3 Immunoenzyme technique
8.4 Avidin-biotin method
8.5 Protein A method
8.6 Immunogold method and immune gold-silver method

Chapter 9 Specificity and Sensitivity of Immunohistochemistry
9.1 Specificity and specific staining
9.2 Control experiment
9.3 The methods to enhance immunohistochemical sensitivity

Chapter 10 The Double-Staining in Immunohistochemistry
10.1 Double-staining Immunohistochemistry on serial section
10.2 Immunofluorescence double labelling technique
10.3 Immunoenzyme double staining technique
10.4 Immunoenzyme-Immunofluorescence double staining
10.5 Immunoenzyme-Immunogold double staining

Chapter 11 The Lectin Histochemistry
11.1 Characteristics and application of lectin
11.2 Application of lectin Histochemistry

Chapter 12 The progress of in Situ Display
12.1 The

Jinsong Zhou, Xi'an Jiaotong University, Shannxi Province, China