Histone Methyltransferases

Not all is SET for methylation: Evolution of eukaryotic protein methyltransferases.- Detection and quantification of histone methyltransferase activity in vitro.-  In Vitro Histone Demethylase Assays.- Preparation and characterization of chromatin templates for histone methylation assays.- Techniques to study automethylation of histone methyltransferases and its functional impact.- Rodriguez Profiling the regulation of histone methylation and demethylation by metabolites and metals.- Determination of histone methyltransferase structure by crystallography.- Determination of histone methyltransferase structures in complex with the nucleosome by cryogenic electron microscopy.- Development and validation of antibodies targeting site-specific histone methylation.- Genetic, genomic and imaging approaches to dissect the role of Polycomb group epigenetic regulators in mice.- Profiling histone methylation in low numbers of cells.-  Automated CUT&RUN using the KingFisher Duo Prime.- Bioinformatics methods for ChIP-seq histone analysis.- Characterization of SET-domain histone lysine methyltransferase substrates using a cofactor S-adenosyl-L-methionine surrogate.- Specificity analysis of protein methyltransferases and discovery of novel substrates using SPOT peptide arrays.- Identifying specific protein interactors of nucleosomes carrying methylated histones using mass spectrometr.- Investigating mitotic inheritance of histone post-translational modifications by triple pSILAC coupled to Nascent Chromatin Capture.- Investigating mitotic inheritance of histone modifications using tethering.- Investigating histone modification dynamics by mechanistic computational modelling.- Screening for small molecule inhibitors of histone methyltransferases.