Human Plasma Proteomics

Exploring the Human Plasma Proteome
Overview of the HUPO Plasma Proteome Project
Data management and preliminary data analysis in the pilot phase of the HUPO Plasma Proteome Project
HUPO Plasma Proteome Project specimen collection and handling: Towards the standardization of parameters for plasma proteome samples
Immunoassay and antibody microarray analysis of the HUPO Plasma Proteome Project reference specimens: Systematic variation between sample types and calibration of mass spectrometry data
Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma
Differences among techniques for high-abundant protein depletion
Immunoaffinity separation of plasma proteins by IgY microbeads: Meeting the needs of proteomic sample preparation and analysis
A novel four-dimensional strategy combining protein and peptide separation methods enables detection of low-abundance proteins in human plasma and serum proteomes
A wide range of protein isoforms in serum and plasma uncovered by a quantitative intact protein analysis system
A study of glycoproteins in human serum and plasma reference standards (HUPO) using multilectin affinity chromatography coupled with RPLC-MS/MS
Evaluation of prefractionation methods as a preparatory step for multidimensional based chromatography of serum proteins
Utility of electrophoretically derived protein mass estimates as additional constraints in proteome analysis of human serum based on MS/MS analysis
Efficient prefractionation of low-abundance proteins in human plasma and construction of a two-dimensional map
Two-dimensional liquid chromatography/tandem mass spectrometry analysis of GradiflowTM fractionated native human plasma
Application of 2-D free-flow electrophoresis/RP-HPLC for proteomic analysis of human plasma depleted of multi high-abundance proteins
Peptidomic analysis of human blood specimens: Comparison between plasma specimens and serum by differential

Gilbert Omenn is Professor of Internal Medicine, Human Genetics, and Public Health at the University of Michigan. He served as Executive Vice President for Medical Affairs and as Chief Executive Officer of the University of Michigan Health System from 1997-2002. He is leader of the international Human Proteome Organization (HUPO) Human Plasma Proteome Project. Omenn is the author of 390 research papers and scientific reviews and author/editor of 17 books. He chaired the presidential/congressional Commission on Risk Assessment and Risk Management ("Omenn Commission"), served on the National Commission on the Environment, and chaired the NAS/NRC/IOM Committee on Science, Engineering and Public Policy.