In Vitro Mutagenesis

Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells.- Mutagenesis and Genome Engineering of Epstein-Barr Virus in Cultured Human Cells by CRISPR/Cas9.- Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice.- All-in-One CRISPR-Cas9/FokI-dCas9 Vector-Mediated Multiplex Genome Engineering in Cultured Cells.- CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-free E8 Medium.- Development of CRISPR/Cas9 for Efficient Genome Editing in Toxoplasma gondii.- Generation of Stable Knockout Mammalian Cells By TALEN-Mediated Locus-Specific Gene Editing.- Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell-

Mediated Semi-Cloned Technology. - Insertion of Group II Intron-Based Ribozyme Switches into Homing Endonuclease Genes.- Generating a Genome Editing Nuclease for Targeted Mutagenesis in Human Cells.- Use of Group II Intron Technology for Targeted Mutagenesis in Chlamydia trachomatis.- in silico Approaches to Identify Mutagenesis Targets to Probe and Alter Protein-Cofactor and Protein-Protein Functional Relationships.- in silico Prediction of Deleteriousness for Non-Synonymous and Splice-Altering Single Nucleotide Variants in the Human Genome.- in silico methods for Analyzing Mutagenesis Targets.- Methods for Detecting Critical Residues in Proteins.- A Method for Bioinformatic Analysis of Transposon Insertion Sequencing (INSeq) Results for Identification of Microbial Fitness Determinants.- Application of in vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea.- Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors.- PERMutation Using Transposase Engineering (PERMUTE):  a Simple Approach for Constructing Circularly-Permuted Protein Libraries.- Transposon Insertion Mutagenesis for Archaeal Gene Discovery.- Genome-Wide Transposon Mutagenesis in Mycobacterium tuberculosis