Introduction to Proteomics

I. Proteomics and the Proteome.- 1. Proteomics and the New Biology.- 2. The Proteome.- II. Tools of Proteomics.- 3. Overview of Analytical Proteomics.- 4. Analytical Protein and Peptide Separations.- 5. Protein Digestion Techniques.- 6. Mass Spectrometers for Protein and Peptide Analysis.- 7. Protein Identification by Peptide Mass Fingerprinting.- 8. Peptide Sequence Analysis by Tandem Mass Spectrometry.- 9. Protein Identification with Tandem Mass Spectrometry Data.- 10. SALSA: An Algorithm for Mining Specific Features of Tandem MS Data.- III. Applications of Proteomics.- 11. Mining Proteomes.- 12. Protein Expression Profiling.- 13. Identifying Protein-Protein Interactions and Protein Complexes.- 14. Mapping Protein Modifications.- 15. New Directions in Proteomics.

"Professor Daniel Liebler presents a tutorial on mass spectrometry and its use in proteomics. The basics of mass spectrometers and ionization techniques are described, which is important to ascertain what type of mass spectrometer is most particular for a particular study. The ability to use mass spectrometry data to search databases is an important advance for the non-specialist, because it no longer requires the development of the skills to interpret mass spectra. A basic understanding of the fundamental of the search algorithms and their limitations is described in the book. Finally, applications of mass spectrometry to proteomics are described. This book provides and excellent introduction and overview of proteomics for the graduate student of for any biologist interested in understanding the basics of this rapidly evolving area."-From the foreword by John R. Yates, III

"...a tutorial of mass spectrometry for the biological sciences in general and more specifically for the field of proteomics,...Although designated as a book for beginners, it will almost certainly find a wider audience. In particular, both academics and researchers working in associated fields with a need to hone their knowledge skills will find much to interest them in this well written book." - Chromatographia

"...an excellent overview of this field in an easy-to-read, succcinct format." - Expert Reviews in Proteomics

"...there is a wonderful description of mass spectrometry of peptides, both simple, accurate, up-to-date and very pleasant to read....This book can be recommended for people needing an update in mass spectrometry in proteomics..." - Proteomics