Lectins

Lectins and Sugars

Kanji Hori and Makoto Hirayama

 

17.     Surface Plasmon Resonance as a Tool to Characterize Lectin-carbohydrate Interactions

Yasuro Shinohara, and Jun-ichi Furukawa

 18.     Isothermal Calorimetric Analysis of Lectin-sugar Interaction



Yoichi Takeda and Ichiro Matsuo

 

19.     Carbohydrate-Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labeled Glycosylasparagines

Mamoru Mizuno

 

 

PART II: EMERGING TECHNIQUES FOR LECTIN-BASED GLYCOMICS

 

20.     Lectin-Based Glycomics: How and When Was the Technology Born?

Jun Hirabayashi

 

21.     Frontal Affinity Chromatography (FAC): Theory and Basic Aspects

Ken-ichi Kasai

 22.     Frontal Affinity Chromatography: Practice of Weak Interaction Analysis Between Lectins and Fluorescently Labeled Oligosaccharides



Chihiro Sato

 

23.     Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation. From Sample Pretreatment to Data Standardization

Atsushi Kuno, Atsushi Matsuda, Sachiko Unno, Binbin Tan, Jun Hirabayashi, and Hisashi Narimatsu

 

24.     Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces

Azusa Shibazaki, and Tohru Gonoi

 

25.      Application of Lectin Microarray to Bacteria Including Lactobacillus casei /paracasei Strains

Emi Yasuda, Tomoyuki Sako, Hiroaki Tateno, and Jun Hirabayashi

 

26.     Live Cell Imaging of Human Pluripotent Stem Cells by a Novel Lectin Probe rBC2LCN

Hiroaki Tateno, Yasuko Onuma, and Yuzuru Ito

 

27.     Carbohydrate-binding Specificity of Lectins Using Multiplexed Glyco-bead Array

Kazuo Yamamoto

 

28.     Supported Molecular Matrix Electrophoresis: A New Membrane Electrophoresis for Characterizing Glycoproteins

Yu-ki Matsuno, and Akihiko Kameyama

 

 

PAPRT III: TECHNIQUES FOR ELUCIDATING FUNCTIONS OF ENDOGENOUS ANIMAL LECTINS

 29.     Overall Strategy for Functional Analysis of Animal Lectins



Norihito Kawasaki

 

30.     Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System

Hiroaki Tateno

 

31.     Potential Usage for in vivo Lectin Screening in Live Animals Utilized by Cell Surface Mimetic Glyco-nanoparticles, Phosphorylcholine-Coated Quantum Dots (PC-QDs)

Maho Amano, Hiroshi Hinou, Risho Miyoshi, and Shin-Ichiro Nishimura

 

32.     Remodeling Cell Surface Glycans using Glycosylation Inhibitors

Norihito Kawasaki

 

33.     Remodeling of Glycans Using Glycosyltransferase Genes

Yuko Naito-Matsui, and Hiromu Takematsu

 

34.     Functional Assay Using Lectin Gene Targeting Technologies (Over-Expression)Motohiro Nonaka, and Toshisuke Kawasaki



 

35.     Analysis of L-selectin-mediated Cellular Interactions under Flow Conditions

Hiroto Kawashima

 

36.     Assessment of weak sugar-binding ability using lectin tetramer and membrane-based glycans

Kazuo Yamamoto

 

 

PART IV: STRUCTURAL BIOLOGY AND ENGINEERING OF LECTINS

 

37.     Perspectives in Glycomics and Lectin Engineering

Jan Tkac, Tomas Bertok, Jozef Nahalka, and Peter Gemeiner

 

38.     Molecular Basis of Pandemic of Avian-Type Influenza Virus

Nongluk Sriwilaijaroen, and Yasuo Suzuki

 

39.     Basic Procedure of X-ray Crystallography for Analysis of Lectin-sugar Interactions

Zui Fujimoto

 

40.     A New Structure Determination Method of Lectins Using a Selenium-containing Sugar Ligand

Hisayoshi Makyio, and Ryuichi Kato

 

41.     NMR Analysis of Carbohydrate-Binding Interactions in Solution: An Approach Using Analysis of Saturation Transfer Difference NMR Spectroscopy

Hikaru Hemmi

 

42.     Small Angle X-ray Scattering to Obtain Models of Multivalent Lectin-Glycan Complexes

Stephen D. Weeks, and Julie Bouckaert

 

43.     Directed Evolution of Lectins by an Improved Error-prone PCR and Ribosome Display Method

Dan Hu, Hiroaki Tateno, and Jun Hirabayashi

 

44.     Tracing Ancestral Specificity of Lectins: Ancestral Sequence Reconstruction Method as a New Approach in Protein Engineering

Tomohisa Ogawa, and Tsuyoshi Shirai

  



PART V: COMPREHENSIVE LISTS

 

45.       Comprehensive List of Lectins: Origins, Natures, and Carbohydrate Specificities

Yuka Kobayashi, Hiroaki Tateno, Haruko Okawa, Kazuo Yamamoto, and Jun Hirabayashi

 

46.     Lectin Structures: Classification Based on the 3-D Structures

Zui Fujimoto, Hiroaki Tateno, and Jun Hirabayashi