Mammalian Oocyte Regulation
Methods and Protocols
(Sprache: Englisch)
This Methods in Molecular Biology(TM) volume offers methodologies enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte substrate uptake, chromosomal segregation and fertilisation.
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This Methods in Molecular Biology(TM) volume offers methodologies enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte substrate uptake, chromosomal segregation and fertilisation.
Klappentext zu „Mammalian Oocyte Regulation “
Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that transforms a primordial germ cell into a mature oocyte (or egg) capable of fertilisation and embryonic development is of unrivalled complexity. The end-product of this remarkable developmental process is the ¿mature¿ egg, now replete with macromolecules and organelles, and poised to support embryonic development if fertilisation should occur. Given the importance of the oocyte for embryonic development, it is of no surprise that the oocyte holds centre stage in fertility clinics and greatly influences the outcome of assisted reproductive treatments (ARTs). Mammalian Oocyte Regulation: Methods and Protocols provides a highly diverse compendium of detailed methodologies - primarily focusing on the murine model but also including chapters on human oocytes - for enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte substrate uptake, chromosomal segregation and fertilisation. Written in the highly successful Methods in Molecular Biology¿ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Mammalian Oocyte Regulation: Methods and Protocols serves as an ideal guide to novice oocyte researchers, experts who wish to add another dimension to their established portfolio, as well as the wider research community.
Inhaltsverzeichnis zu „Mammalian Oocyte Regulation “
1. Studying Recombination in Mouse OocytesXianfei Sun and Paula E. Cohen 2. Analysis of Recombinants in Female Mouse MeiosisEsther de Boer, Maria Jasin, and Scott Keeney 3. Studying Meiosis Specific Cohesins in Mouse Embryonic OocytesJi-hye Kim, Kei-ichiro Ishiguro, Nobuaki Kudo, and Yoshinori Watanabe 4. Immunohistochemical Approaches to the Study of Human Fetal Ovarian DevelopmentJing He, Andrew J. Childs, Jieqian Zhou, and Richard A. Anderson 5. Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early EmbryosJacek Z. Kubiak 6. Time-Lapse Epi-Fluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse OocytesJanet E. Holt, Simon I.R. Lane, and Keith T. Jones 7. Using FRET to Study RanGTP Gradients in Live Mouse OocytesJulien Dumont and Marie-Hélène Verlhac 8. Making cRNA for Microinjection and Expression of Fluorescently-Tagged Proteins for Live-Cell Imaging in OocytesMark Levasseur 9. RNAi-Based Methods for Gene Silencing in Mouse OocytesPaula Stein, Petr Svoboda, and Richard M. Schultz 10. Micro-Injection of Morpholino Oligonucleotides for Depleting Securin in Mouse OocytesPetros Marangos 11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and EmbryosJay M. Baltz, Hannah E. Corbett, and Samantha Richard 12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer 13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse OocytesKuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang 14. Chromosome Spreads with Centromere Staining in Mouse OocytesJean-Philippe Chambon, Khaled Hached, and Katja Wassmann 15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy Lynne
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Anguish and Scott Coonrod 16. Measuring Ca2+ Oscillations in Mammalian EggsKarl Swann 17. Counting Chromosomes in Intact EggsTeresa Chiang and Michael A. Lampson 18. Free-Hand Bisection of Mouse Oocytes and EmbryosZbigniew Polanski and Jacek Z. Kubiak 19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and OocytesSouraya Jaroudi and Dagan Wells 20. Nuclear Transfer in the Mouse OocyteEiji Mizutani, Atsuo Ogura, and Teruhiko WakayamaPetros Marangos 11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and EmbryosJay M. Baltz, Hannah E. Corbett, and Samantha Richard 12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer 13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse OocytesKuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang 14. Chromosome Spreads with Centromere Staining in Mouse OocytesJean-Philippe Chambon, Khaled Hached, and Katja Wassmann 15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy Lynne Anguish and Scott Coonrod 16. Measuring Ca2+ Oscillations in Mammalian EggsKarl Swann 17. Counting Chromosomes in Intact EggsTeresa Chiang and Michael A. Lampson 18. Free-Hand Bisection of Mouse Oocytes and EmbryosZbigniew Polanski and Jacek Z. Kubiak 19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and OocytesSouraya Jaroudi and Dagan Wells 20. Nuclear Transfer in the Mouse OocyteEiji Mizutani, Atsuo Ogura, and Teruhiko WakayamaPetros Marangos 11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and EmbryosJay M. Baltz, Hannah E. Corbett, and Samantha Richard 12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer 13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse OocytesKuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang 14. Chromosome Spreads with Centromere Staining in Mouse OocytesJean-Philippe Chambon, Khaled Hached, and Katja Wassmann 15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy Lynne Anguish and Scott Coonrod 16. Measuring Ca2+ Oscillations in Mammalian EggsKarl Swann 17. Counting Chromosomes in Intact EggsTeresa Chiang and Michael A. Lampson 18. Free-Hand Bisection of Mouse Oocytes and EmbryosZbigniew Polanski and Jacek Z. Kubiak 19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and OocytesSouraya Jaroudi and Dagan Wells 20. Nuclear Transfer in the Mouse OocyteEiji Mizutani, Atsuo Ogura, and Teruhiko WakayamaPetros Marangos 11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and EmbryosJay M. Baltz, Hannah E. Corbett, and Samantha Richard 12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer 13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse OocytesKuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang 14. Chromosome Spreads with Centromere Staining in Mouse OocytesJean-Philippe Chambon, Khaled Hached, and Katja Wassmann 15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy Lynne Anguish and Scott Coonrod 16. Measuring Ca2+ Oscillations in Mammalian EggsKarl Swann 17. Counting Chromosomes in Intact EggsTeresa Chiang and Michael A. Lampson 18. Free-Hand Bisection of Mouse Oocytes and EmbryosZbigniew Polanski and Jacek Z. Kubiak 19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and OocytesSouraya Jaroudi and Dagan Wells 20. Nuclear Transfer in the Mouse OocyteEiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama
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Bibliographische Angaben
- 2012, XI, 311 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Hayden A. Homer
- Verlag: Springer, Berlin
- ISBN-10: 1627031901
- ISBN-13: 9781627031905
- Erscheinungsdatum: 09.11.2012
Sprache:
Englisch
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