Methods in Endothelial Cell Biology
(Sprache: Englisch)
Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell...
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Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions.
Methods in Endothelial Cell Biology is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field.
Inhaltsverzeichnis zu „Methods in Endothelial Cell Biology “
Part I: Isolation and Culture of Endothelial Cells Chapter 1 Isolation, Purification, and Culture of Human Micro- and Macrovascular Endothelial CellsNancy Laurens andVictor W.M. van Hinsbergh Chapter 2 Isolation of Mouse Cardiac Microvascular Endothelial CellsRalf P. Brandes, Stefani Beer, and Alexandra Aicher Chapter 3 Isolation,Culture, and Differentiation of Circulating Endothelial Precursor CellsCarmen Urbich and Stefanie Dimmeler Chapter 4 Embryonic Stem Cell-Derived Endothelial CellsJun Yamashita and Shin-Ichi Nishikawa Chapter 5 Detection, Isolation, and Culture Of Lymphatic Endothelial CellsTaija Mäkinen and Kari Alitalo Chapter 6 Three-Dimensional Spheroid Culture of Endothelial CellsThomas Korff Chapter 7 Immortalization of Endothelial CellsYvonne Reiss and Friedemann Kiefer Chapter 8 Viral Gene Transfer Into Endothelial CellsChristoph Volpers and Stefan Kochanek Part II: Functional Assays in Vitro Chapter 9 Leukocyte Adhesion to Endothelium in Vitro Under Shear StressOlaf Zille, Markus Hammel, and Rupert Hallmann Chapter 10 Application of Biomechanical Forces on Endothelial CellsAndreas Schubert and Henning Morawietz Chapter 11 Endothelial Cell Permeability Assays in CultureMaria Grazia Lampugnani and Elisabetta Dejana Chapter 12 Three-Dimensional in Vitro Angiogenesis AssaysThomas Korff Chapter 13 Rat Aortic Ring Assay of AngiogenesisRoberto F. Nicosia and Wen-Hui Zhu Chapter 14 Endothelial Cell Chemotaxis and Chemokinesis AssaysHellmut G.Augustin Chapter 15 FACS Analysis of Endothelial CellsYvonne Reiss and Britta Engelhardt Chapter 16 Application of the RNA Interference (RNAi) Technology to Angiogenesis ResearchSung-Suk chae, Ji-Hye Paik, Jonathan Shubert-Coleman, Henry Furneaux, andTimothy Hla Part III: Functional Assays in Vivo Chapter 17 Murine Bone Marrow Transplantation Models that Enable the Study of EPC RecruitmentToshinori Murayama,Oren M. Tepper, and Takayuki Asahara Chapter 18 Balloon Denudation of Blood VesselsVolkhard Lindner Chapter 19
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Peripheral Hind Limb Ischemia ModelsStephan Schirmer, Imo Hoefer, and Ivo Buschmann Chapter 20 In Vivo Matrigel Angiogenesis AssaysTony Passaniti and Michele I.Vitolo Chapter 21 Analysis of Vascular Permeability in vivoKatja Issbrücker,Hugo H.Marti, and Matthias Clauss Chapter 22 Validating the Manipulation of Endothelial Cell signaling in VivoJohn S. Rudge and Gavin Thurston Chapter 23 Chick Chorioallantoic Membrane Assay: Growth Factor and Tumor-induced Angiogenesis and LymphangiogenesisMartin Hagedorn and Jörg Wilting Chapter 24 Corneal Angiogenesis AssayLucia Morbidelli, Silvia Cantara, and Marina Ziche Part IV: Detection of Endothelial Cells Chapter 25 Basic Blood Vessel ImmunohistochemistryCynthia Obobodozie and Jens Kroll Chapter 26 Double-Staining Immunohistochemistry of Blood VesselsValentin Goede Chapter 27 In Situ Hybridization Analysis of Vascular EndotheliumRegina Heidenreich and Georg Breier Chapter 28 High-Resolution in Situ Confocal Analysis of Endothelial CellsHolger Gerhardt and Christer Betsholtz Chapter 29 Whole Mount Analysis of the Embryonic VasculatureJody J.Haigh and Andras Nagy Chapter 30 Methods for Visualizing Intact Blood VesselsGavin Thurston and Danielle Jean-Guillaume Chapter 31 Intravital Multi-Fluorescence MicroscopyMohammad Farhadi, Karsten Schwinn, and Peter Vajkoczy Chapter 32 Corrosion Cast Analysis of Blood VesselsValentin Djonov and Peter H.
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Bibliographische Angaben
- 2004, 383 Seiten, mit farbigen Abbildungen, Maße: 15,5 x 23,5 cm, Kartoniert (TB), Englisch
- Herausgegeben:Augustin, Hellmut G.
- Herausgegeben: Hellmut G. Augustin
- Verlag: Springer
- ISBN-10: 354021397X
- ISBN-13: 9783540213970
- Erscheinungsdatum: 20.07.2004
Sprache:
Englisch
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