Methods in Methane Metabolism, Part B

1. Characterization of the Recombinant Pyrophosphate-Dependent 6-Phosphofructokinases from Methylomicrobium alcaliphilum 20Z and Methylococcus capsulatus Bath 2. Genes and Enzymes of Ectoine Biosynthesis in Halotolerant Methanotrophs 3. Facultative And Obligate Methanotrophs: How To Identify And Differentiate Them 4. Approaches for the Characterization and Description of Novel Methanotrophic Bacteria 5. Methylococcus capsulatus (Bath): From Genome to protein function, and vice versa 6. Functional metagenomics of methylotrophs 7. Genetic Systems for Moderately Halo(alkali)philic Bacteria of the genus Methylomicrobium 8. Development of a system for genetic manipulation of the facultative methanotroph Methylocella silvestris BL2 9. Mutagenesis of soluble methane monooxygenase 10. Single cell methods for methane oxidation analysis 11. Methanotroph Outer Membrane Preparation 12. Over-Expression And Purification Of The Particulate Methane Monooxygenase From Methylococcus Capsulatus(Bath) 13. Metal reconstitution of particulate methane monooxygenase and heterologous expression of the pmoB subunit 14. Particulate Methane Monooxygenase From Methylosinus Trichosporium Ob3b 15. Production, Isolation, Purification and Functional Characterisation of Methanobactins 16. A simple assay for screening microorganisms for chalkophore production 17. Isolation of Methanobactin From the Spent Media of Methane Oxidizing Bacteria 18. Measurements of biosphere-atmosphere exchange of CH4 in terrestrial ecosystems