Molecular and Cell Biology Methods for Fungi

Fungal Transformation and Gene Knockout.- Protoplast Transformation of Filamentous Fungi.- Electroporation and Agrobacterium-Mediated Spore Transformation.- High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains.- Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi.- DelsGate: A Robust and Rapid Method for Gene Deletion.- Gene Silencing for Functional Analysis: Assessing RNAi as a Tool for Manipulation of Gene Expression.- Detection and Quantification of Fungi.- Analysis of Fungal Gene Expression by Real Time Quantitative PCR.- Identification of Differentially Expressed Fungal Genes In Planta by Suppression Subtraction Hybridization.- Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software.- Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR.- Application of Laser Microdissection to Study Plant-Fungal Pathogen Interactions.- Multiplex Gene Expression Analysis by TRAC in Fungal Cultures.- Amplification of Fungal Genomes Using Multiple Displacement Amplification.- Microscopy and Protein Analysis.- Biochemical Methods Used to Study the Gene Expression and Protein Complexes in the Filamentous Fungus Neurospora crassa.- Measuring Protein Kinase and Sugar Kinase Activity in Plant Pathogenic Fusarium Species.- A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae.- A Method to Visualize the Actin and Microtubule Cytoskeleton by Indirect Immunofluorescence.- Fluorescence In Situ Hybridization for Molecular Cytogenetic Analysis in Filamentous Fungi.- Live-Cell Imaging of Microtubule Dynamics in Hyphae of Neurospora crassa.- Methods to DetectApoptotic-Like Cell Death in Filamentous Fungi.- Evaluation of Antifungal Susceptibility Using Flow Cytometry.- Preparation of Fungi for Ultrastructural Investigations and Immunogoldlabelling.- Split-EGFP

From the reviews:"This volume of methods and step-by-step protocols for fungal molecular biology deals with three areas: (i) fungal transformation and gene knockout; (ii) detection and quantitation of fungi; and (iii) microscopy and protein analysis. The authors of the chapters are world leaders in their respective fields and the book ... still a valuable laboratory resource. ... this is a good place for a freshman fungal lab worker to start - and it also provides a useful list of contacts who no doubt could field further questions." (Neil A. R. Gow, Microbiology Today, 2010)