Molecular Fluorescence
Principles and Applications
(Sprache: Englisch)
This second edition of the well-established bestseller is completely updated and revised with approximately 30% additional material, including two new chapters on applications, which has seen the most significant developments.
The comprehensive overview...
The comprehensive overview...
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Produktinformationen zu „Molecular Fluorescence “
This second edition of the well-established bestseller is completely updated and revised with approximately 30% additional material, including two new chapters on applications, which has seen the most significant developments.
The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips.
For complex, physical, food and photochemists, molecular physicists, lecturers and students of chemistry and physics, and molecular biologists.
The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips.
For complex, physical, food and photochemists, molecular physicists, lecturers and students of chemistry and physics, and molecular biologists.
Klappentext zu „Molecular Fluorescence “
This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material,including two new chapters on applications, which has seen the most signifi cant developments.The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips.For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturersand students of chemistry, physics, and biology.
Inhaltsverzeichnis zu „Molecular Fluorescence “
INTRODUCTIONWhat is Luminescence?A Brief History of Fluorescence and PhosphorescencePhotoluminescence of Organic and Inorganic Species: Fluorescence or Phosphorescence?Various De-Excitation Processes of Excited MoleculesFluorescent Probes, Indicators, Labels, and TracersUltimate Temporal and Spatial Resolution: Femtoseconds, Femtoliters, Femtomoles, and Single-Molecule DetectionPART I: PrinciplesABSORPTION OF ULTRAVIOLET, VISIBLE, AND NEAR-INFRARED RADIATIONElectronic TransitionsTransition Probabilities: The Beer-Lambert Law, Oscillator StrengthSelection RulesThe Franck-Condon PrincipleMultiphoton Absorption and Harmonic GenerationCHARACTERISTICS OF FLUORESCENCE EMISSIONRadiative and Nonradiative Transitions between Electronic StatesLifetimes and Quantum YieldsEmission and Excitation SpectraSTRUCTURAL EFFECTS ON FLUORESCENCE EMISSIONEffects of the Molecular Structure of Organic Molecules on Their FluorescenceFluorescence of Conjugated Polymers (CPs)Luminescence of Carbon Nanostructures: Fullerenes, Nanotubes, and Carbon DotsLuminescence of Metal Compounds, Metal Complexes, and Metal ClustersLuminescence of Semiconductor Nanocrystals (Quantum Dots and Quantum Rods)ENVIRONMENTAL EFFECTS OF FLUORESCENCE EMISSIONHomogeneous and Inhomogeneous Band Broadening-Red-Edge EffectsGeneral Considerations on Solvent EffectsSolvent Relaxation Subsequent to Photoinduced Charge Transfer (PCT)Theory of Solvatochromic ShiftsEffects of Specific InteractionsEmpirical Scales of Solvent PolarityViscosity EffectsFluorescence in Solid Matrices at Low TemperatureFluorescence in Gas Phase: Supersonic JetsEFFECTS OF INTERMOLECULAR PHOTOPHYSICAL PROCESSES ON FLUORESCENCE EMISSIONIntroductionOverview of the Intermolecular De-Excitation Processes of Excited Molecules Leading to Fluorescence QuenchingPhotoinduced Electron TransferFormation of Excimers and ExciplexesPhotoinduced Proton TransferFLUORESCENCE POLARIZATION: EMISSION ANISOTROPHYPolarized LIght and Photoselection of Absorbing
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MoleculesCharacterization of the Polarization State of Fluorescence (Polarization Ratio and Emission Anisotropy)Instantaneous and Steady-State AnisotropyAdditivity Law of AnisotropyRelation between Emission Anisotropy and Angular Distribution of the Emission Transition MomentsCase of Motionless Molecules with Random OrientationEffect of Rotational MotionApplicationsEXCITATION ENERGY TRANSFERIntroductionDistiction between Radiative and Nonradiative TransferRadiative Energy TransferNonradiative Energy TransferDetermination of Distances at a Supramolecular Level Using FRETFRET in Ensembles of Donors and AcceptorsFRET between Like Molecules: Excitation Energy Migration in Assemblies of ChromophoresOverview of Qualitative and Quantitative Applications of FRETPART II: TechniquesSTEADY-STATE SPECTROFLUOROMETRYOperating Principles of a SpectrofluorometerCorrection of Excitation SpectraCorrection of Emission SpectraMeasurement of Fluorescence Quantum YieldsPossible Artifacts in SpectrofluorometryMeasurement of Steady-State Emission Anisotropy: Polarization SpectraTIME-RESOLVED FLUORESCENCE TECHNIQUESBasic Equations of Pulse and Phase-Modulation FluorimetriesPulse FluorimetryPhase-Modulation FluorimetryArtifacts in Time-Resolved FluorimetryData AnalysisLifetime StandardsTime-Resolved Polarization MeasurementsTime-Resolved Fluorescence SpectraLifetime-Based Decomposition of SpectraComparison between Single-Photon Timing Fluorimetry and Phase-Modulation FluorimetryFLUORESCENCE MICROSCOPYWide-Field (Conventional), Confocal, and Two-Photon Fluorescence MicroscopiesSuper-Resolution (Subdiffraction) TechniquesFluorescence Lifetime Imaging Microscopy (FLIM)ApplicationsFLUORESCENCE CORRELATION SPECTROSCOPY AND SINGLE-MOLECULE FLUORESCENCE SPECTROSCOPYFluorescence Correlation Spectroscopy (FCS)Single-Molecule Fluorescence SpectroscopyPART III: ApplicationsEVALUATION OF LOCAL PHYSICAL PARAMETERS BY MEANS OF FLUORESCENT PROBESFluorescent Probes for PolarityPyrene and Its DerivativesEstimation of "Microviscosity", Fluidity, and Molecular MobilityTemperaturePressureCHEMICAL SENSING VIA FLUORESCENCEIntroductionVarious Approaches of Fluorescence SensingFluorescent pH IndicatorsDesign Principles of Fluorescent Molecular Sensors Based on Ion or Molecule RecognitionFluorescent Molecular Sensors of Metal IonsFluorescent Molecular Sensors of AnionsFluorescent Molecular Sensors of Neutral MoleculesFluorescence Sensing of GasesSensing DevicesRemote Sensing by Fluorescence LIDARAUTOFLUORESCENCE AND FLUORESCENCE LABELING IN BIOLOGY AND MEDICINEIntroductionNatural (Intrinsic) Chromophores and FluorophoresFluorescent Proteins (FPs)Fluorescent Small MoleculesQuantum Dots and Other Luminescent NanoparticlesConclusionMISCELLANEOUS APPLICATIONSFluorescent Whitening AgentsFluorescent Nondestructive TestingFood ScienceForensicsCounterfeit DetectionFluorescence in ArtAPPENDIXCharacteristics of Fluorescent Compounds
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Autoren-Porträt von Bernard Valeur, Mário N. Berberan-Santos
Bernard Valeur received his undergraduate and PhD degrees at Ecole Sup#;rieure de Physique et de Chimie Industrielles de Paris (E.S.P.C.I.). He was a postdoctoral fellow at the University of Illinois at Urbana-Champaign. After being an associate professor at the Ecole Supérieure de Physique et Chimie Industrielles de Paris, he became full professor of physical chemistry at the Conservatoire National des Arts et Métiers, where he is emeritus professor since 2008. He has authored over 150 publications and is a member of several editorial boards.Mario Berberan-Santos is a Senior Researcher at the Instituto Superior Técnico (IST) at the Technical University of Lisbon. He graduated in Chemical Engineering from IST in 1984. After a brief stay at the National Research Council of Canada (Ottawa), he received his Ph. D. in Chemistry from IST in 1989. He was a post-doctoral Fellow at Conservatoire National des Arts et Métiers (Paris, France), and at Laboratoire pour l'Utilisation du Rayonnement Electromagnétique (LURE, Univ. Paris-Sud, Orsay, France). He has been Invited Professor of Physical Chemistry at Universidade do Algarve (Faro, Portugal) and Invited Full Professor at the École Normale Supérieure de Cachan (France). He has authored 135 articles in scientific journals and 13 book chapters. He is member of several editorial advisory boards and Vice-President of the Portuguese Chemical Society.s Arts et Métiers, where he is emeritus professor since 2008. He has authored over 150 publications and is a member of several editorial boards.
Mario Berberan-Santos is a Senior Researcher at the Instituto Superior Técnico (IST) at the Technical University of Lisbon. He graduated in Chemical Engineering from IST in 1984. After a brief stay at the National Research Council of Canada (Ottawa), he received his Ph. D. in Chemistry from IST in 1989. He was a post-doctoral Fellow at Conservatoire National des Arts et Métiers (Paris, France), and at Laboratoire pour l'Utilisation
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du Rayonnement Elec
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Bibliographische Angaben
- Autoren: Bernard Valeur , Mário N. Berberan-Santos
- 2012, 2. Aufl., XXI, 569 Seiten, 22 farbige Abbildungen, 225 Schwarz-Weiß-Abbildungen, Maße: 16,9 x 24,6 cm, Gebunden, Englisch
- Verlag: Wiley-VCH
- ISBN-10: 3527328378
- ISBN-13: 9783527328376
- Erscheinungsdatum: 04.05.2012
Sprache:
Englisch
Rezension zu „Molecular Fluorescence “
"Molecular Fluorescence is a comprehensive textbook and reference book that provides a deep and critical analysis of this evolving field." ( Journal of Biomedical Optics , 1 March 2013) "The strength of the book lies in its clear and understandable presentation, and in the thoroughness of the descriptions of fluorescence applications, enabling one to quickly appreciate the many questions and problems in the field of fluorescence. Molecular Fluorescence is more a textbook than a monograph, and therefore it is of special interest for students and beginners in the field, and be recommended." - Angewandte Chemie (international edition), 2002; Vol. 41 No. 16
Pressezitat
"The strength of the book lies in its clear and understandable presentation, and in the thoroughness of the descriptions of fluorescence applications, enabling one to quickly appreciate the many questions and problems in the field of fluorescence. Molecular Fluorescence is more a textbook than a monograph, and therefore it is of special interest for students and beginners in the field, and be recommended."- Angewandte Chemie (international edition), 2002; Vol. 41 No. 16
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