Neurotrophic Factors

1. Neurotrophic Factors: An Overview.- Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions.- Culture of Rodent Cortical, Hippocampal, and Striatal Neurons.- Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex, Spinal Cord, and Cerebellum.- Central Nervous System Neuron-Glia Co-Culture Models and Application to Neuroprotective Agents.- Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assays.- Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening.- Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotective Agents.- Culture of Neurons and Smooth Muscle Cells from the Myenteric Plexus of Adult Mice.- Astrocyte/Microglia Co-Cultures as a Model to Study Neuroinflammation.- Micropatterned Geometries Shape Oligodendrocyte and Microglia Plasticity.- Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repair.- Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neonatal Rodent Microglia.- Diolistic Labeling and Analysis of Dendritic Spines.- Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses.- Methods for Quantitative Analysis of Axonal Cargo Transport.- Neuronal Cell Morphology in Primary Cerebellar Granule Cells Using High-Content Analysis.- Nerve Growth Factor-Induced Angiogenesis: 1. Endothelial Cell Tube Formation Assay.- Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay.- Rat Pancreatic Beta-Cell Culture.- Growth and Neurotrophic Factors in Embryonic Stem Cells.- Site-Specific Direct Labeling of Neurotrophins and Their Receptors: From Biochemistry to Advanced Imaging Applications.- Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro