PCR Topics

Basic Methodology and Research Applications.- Quantitation of mRNA by the Polymerase Chain Reaction.- Sequencing of PCR Products - Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes.- Alternative Methods for DNA Probing in Diagnosis: Ligase Chain Reaction (LCR).- Application of PCR to the Detection and Analysis of Point Mutations in the Human Factor VIII Gene.- The Use of PCR in the Diagnosis of Muscle Disease.- Taq DNA Polymerase-Synthesized Single-Stranded DNA Hybridization Probes and their Application in Northern Blotting and in situ Hybridization.- Computer Program for the Selection of PCR Primers.- Nervous System Cross Reactivity Sequences.- The Use of Short Adapters for Priming PCR of Unknown Chromosomal DNA Fragments.- Labeling of Specific DNA Sequences With Digoxigenin during Polymerase Chain Reaction.- Quantitative Analysis of Polymerase Chain Reaction (PCR) Products by Means of Fluorescence Detection.- Reverse Transcription PCR (RT-PCR) of Parathyroid Hormone (PTH) mRNA.- False Positive Reactions in PCR.- Error Production and Error Propagation During PCR.- Genetics and Evolution.- PCR in Linkage Analysis of Genetic Diseases.- Amplification, Isolation and Direct Nucleotide Determination of Entire Genes: Application to the Study of 16S rRNAs for Molecular Evolution in Bacteria, Identification of Cultural Isolates and Development of Probes.- Diagnosis of Cystic Fibrosis - Detection of the Gene Deletion Delta F 508.- Application of PCR for Prenatal Diagnosis of Cystic Fibrosis (CF).- The Use of Non-Viable Cultured Amniotic Fluid Cells for Prenatal Diagnosis of Cystic Fibrosis by Polymerase Chain Reaction.- Determination of Glutathione S-Transferase u Genotype in Humans by PCR-Amplified Gene-Probes.- Rapid Apolipoprotein E GenotypeIdentification.- Detection and Characterization of Viral Infections.- Herpes Viruses.- Detection of Herpes Viruses by PCR - Diagnostic Aspects.- Diagnosis of Herpes Simplex Virus Encephalitis (HSVE) by