Peptide Characterization and Application Protocols

Peptide Characterization and Application Protocols Preface Contributors Section 1: Characterization Chapter 1: HPLC Analysis and Purification of Peptides Colin T. Mant, Yuxin Chen, Zhe Yan, Traian V. Popa, James M. Kovacs, Janine B. Mills, Brian P. Tripet, and Robert S. Hodges Chapter 2: Identification of Proteins Based on MS/MS Spectra and Location of Post-translational Modifications Kathryn L. Stone, Myron Crawford, Walter McMurray, Nancy Williams, and Kenneth R. Williams Chapter 3: Determination of Phosphorylated and O-glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature Gary M. Hathaway Chapter 4: Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein-Coupled Receptor Fred Naider Section 2: Application Chapter 5: Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors Janelle L. Lauer-Fields, Dmitriy Minond, Keith Brew, and Gregg B. Fields Chapter 6: Proteolytic Profiling of the Extracellular Matrix Degradome Diane Baronas-Lowell, Janelle L. Lauer-Fields, Mohammad Al-Ghoul, and Gregg B. Fields Chapter 7: s-Amyloid Protein Aggregation Inhibition Marcus A. Etienne, Nadia J. Edwin, Jed P. Aucoin, Paul S. Russo, Robin L. McCarley, and Robert P. Hammer Chaper 8: Radiometal-labeled Somatostatin Analogues for Applications in Cancer Imaging and Therapy Jason S. Lewis and Carolyn J. Anderson Chapter 9: Cell Penetrating Proline Rich Peptidomimetics Josep Farrera-Sinfreu, Ernest Giralt, Miriam Royo, and Fernando Albericio Chapter 10: Peptide-Mediated Targeting of Liposomes to Tumor Cells Evonne M. Rezler, David R. Khan, Raymond Tu, Matthew Tirrell, and Gregg B. Fields Chapter 11: Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells Sébastien Deshayes, Federica Simeoni, May C. Morris, Gilles Divita, and FrédéricHeitz Chaper 12: Broth Microdilution Antibacterial Assay of Peptides Laszlo Otvos and Mare Cudic Chapter 13: Depsipeptide Synthesis Maciej Stawikowski and Predrag Cudic