Plant Cells and their Organelles

Contributors ix
 
Preface xi
 
Acknowledgments xii
 
1 An introduction to cells and their organelles 1
William V. Dashek
 
Cells 1
 
Cell organelles - an introduction 6
 
Ion channels 10
 
Proton pumps 14
 
Water channels 14
 
Carriers 15
 
Cell death 17
 
References 18
 
Further reading 24
 
2 Isolation and characterization of subcellular organelles from plant cells 25
Milee Agarwal, P. Desai, and Harish Padh
 
Isolation of subcellular organelles 26
 
Identification and characterization of isolated organelles 33
 
Summary 39
 
References 39
 
Further reading 41
 
3 Endoplasmic reticulum 42
William V. Dashek
 
Structure 42
 
Chemical composition 42
 
Biogenesis 45
 
Functions 45
 
Posttranslational events 49
 
Inhibitors 53
 
In vitro protein synthesis 54
 
Other functions 54
 
References 54
 
Further reading 60
 
4 The Golgi apparatus 61
D. Davis, T.E. Wilkop, and Georgia Drakakaki
 
The Golgi apparatus 61
 
Plant Golgi introduction 66
 
Structure and organization 69
 
Golgi-mediated vesicular trafficking 71
 
Plant Golgi-dependent cellular processes 74
 
Imaging and visualization 76
 
Isolation and analysis 78
 
Golgi genetics and genomics 81
 
Significance 84
 
Acknowledgment 85
 
References 85
 
Further reading 87
 
5 Microbodies 88
Robert Donaldson
 
Introducing peroxisomes 88
 
Leaf peroxisomes 89
 
Peroxisomes in oil seeds and pollen 91
 
References 107
 
Further reading 109
 
6 Microtubules, intermediate filaments, and actin filaments 110
William V. Dashek
 
Microtubules 110
 
Intermediate filaments 113
 
Actin filaments (microfilaments) 116
 
References 119
 
Further reading

About the Editors
William V. Dashek, Emeritus Professor, Mary Baldwin College, USA
Dr. Dashek is the recipient of BS, MS and Ph.D. degree from Marquette University where he was a National Institute of Health pre-doctoral trainee. He was a post-doctorate fellow at the Michigan State University Plant research Laboratory. Subsequently, he began a lengthy Academic teaching and research career. Currently, Dashek is retired writing advanced textbooks.
 
Gurbachan S. Miglani, Visiting Professor, School of Agricultural Biotechnology, Punjab Agricultural University, India
Dr. Miglani obtained B.Sc. (Agri. & A.H.) and M.Sc. (Genetics) from Punjab Agricultural University (PAU), Ludhiana, Punjab, India and Ph.D. from Howard University, Washington, D.C. During his 35-year teaching stint at PAU he taught general and specialized courses in genetics. Even after retirement Miglani is still teaching molecular genetics and biotechnology at PAU. In addition to research and review papers, popular science articles, laboratory manuals he has published a dozen books and presently he is writing his next book.