Post-Transcriptional Gene Regulation

Table of Contents Preface Contributors 1. Bioinformatics Approaches for Studying Untranslated Regions of mRNAs Paramjeet S. Bagga 2. Identification of mRNA Polyadenylation Sites in genomes Using cDNA Sequences, Expressed Sequence Tags and Trace Ju Youn Lee, Ji Yeon Park and Bin Tian 3. Bioinformatic Tools for Studying Post-Transcriptional Gene Regulation: The UAlbany TUTR (Training UTR) Collection and other Informatic Resources Francis Doyle, Christopher Zaleski, Alish D. George, Eric Stenson, Adele Ricciardi and Scott A. Tenenbaum 4. In-Line Probing Analysis of Riboswitches Elizabeth E. Regulski and Ronald R. Breaker 5. Ribotrap: Targeted Purification of RNA-Specific RNPs from Cell Lysates via Immunoaffinity Precipitation to Identify Regulatory Proteins and RNAs Dale L. Beach and Jack D. Keene 6. Advances in RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation- Microarray (Chip) Profiling Timothy E. Baroni, Sridar V. Chittur, Ajish D. George and Scott A. Tenenbaum 7. Biosensors for RNA Aptamer-Protein Interaction Sara Tombelli, Maria Minunni and Marco Mascini 8. A Tethering Approach to Study Proteins that Activate mRNA Turnover in Human Cells Sandra L. Clement and Jens Lykke-Andersen 9. RNA Analysis by Biosynthetic Tagging (RABT) Using 4-Thiouracil and Uracil Phosphoribosyltransferase Gusti M. Zeiner, Michael D. Cleary, Ashley E. Fotus, Christopher D. Meiring, Edward S. Mocarski and John C. Boothroyd 10. Efficient 5' Cap Dependent RNA Purification: Use in Identifying and Studying Subsets of RNA Edyta Z. Bajak and Curt H. Hagedorn 11. Enrichment of Alternatively Spliced Isoforms (EASI) Julian P. Venables 12. In Vivo Methods to Assess Polyadenylation Efficiency Lisa K. Hague, Tyra Hall-Pogarand Carol S. Lutz 13. Monitoring the Temporal and Spatial Distribution of RNA in Living Yeast Cells Roy M. Long and Carl R. Urbinati 14. Analysis of mRNA Partitioning Between the Cytosol and Endoplasmic Reticulum Compartments of Mammalian Cells Samuel B. Stephens, Rebecca D.