Protein Acetylation

Validation of Protein Acetylation by Mass Spectrometry.- Application of the CIRAD Mass Spectrometry Approach for Lysine Acetylation Site Discovery.- Application of the MIDAS Approach for Analysis of Lysine Acetylation Sites.- Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein Acetylations.- Towards the N-terminal Acetylome: An N-terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose Resin.- Identification and Analysis of O-acetylated Sialoglycoproteins.- HPLC-based Quantification of in vitro N-terminal Acetylation.- Separation and Purification of Multiply Acetylated Proteins using Cation-exchange Chromatography.- In-gel N-acetylation for the Quantification of the Degree of Protein in vivo N-terminal Acetylation.- Computational Prediction of Lysine Acetylation Proteome-wide.- Generation and Characterization of Pan-specific Anti-acetyllysine Antibody.- Using Functional Proteome Microarrays to Study Protein Lysine Acetylation.- Quantitation of Nucleosome Acetylation and other Histone Post-Translational Modifications Using Microscale NU-ELISA.- Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core.- Production of Amino-terminally Acetylated Recombinant Proteins in E. coli.- Identification of Lysine Acetyltransferase Substrates using Bioorthogonal Chemical Proteomics.- Non-radioactive in-vitro Assays for Histone Deacetylases.- The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive Probes.- Analysis of Protein Acetyltransferase Structure-function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule Modulators.