Protein Acetylation

High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets.- Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry.- Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it Chemisty.- Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC.- Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In Vivo.- Site-Specific Lysine Acetylation Stoichiometry across Subcellular Compartments.- Lysine Acetylation of Proteins and its Characterization in Human Systems.- Molecular and Cellular Characterization of SIRT1 Allosteric Activators.- Assessment of SIRT2 Inhibitors in Mouse Models of Cancer.- Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair.- Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes.- Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine Acetylation.- Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons.- Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability.- Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling.- Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroepigenetics.- Functional Analysis of HDACs in Tumorigeneis.