Protein Engineering Handbook.Vol.3

PREFACE DIRIGENT EFFECTS IN BIOCATALYSIS Introduction Dirigent Proteins Solvents and Unconventional Reaction Media Structure and Folding Structured and Unstructured Domains Isozymes, Moonlighting Proteins, and Promiscuity: Supertalented Enzymes Conclusions PROTEIN ENGINEERING GUIDED BY NATURAL DIVERSITY ApproachesProtocols Future Directions Conclusions PROTEIN ENGINEERING USING EUKARYOTIC EXPRESSION SYSTEMS Introduction Eukaryotic Expression Systems Conclusions PROTEIN ENGINEERING IN MICRODROPLETS IntroductionDroplet Formats Perspectives FOLDING AND DYNAMICS OF ENGINEERED PROTEINS IntroductionProof-of-Principle Protein Designs Proteins Designed for Function Conclusions and Outlook ENGINEERING PROTEIN STABILITY Introduction Power and Scope of Protein Engineering to Enhance Stability Measurement of a Protein's Kinetic Stability Developments in Protein Stabilization ENZYMES FROM THERMOPHILIC ORGANISMS Introduction Hyperthermophiles Enzymes from Thermophiles and Their Reactions Production of Proteins from (Hyper)Thermophiles Protein Engineering of Thermophilic Proteins Cell Engineering in Hyperthermophiles Future Perspectives ENZYME ENGINEERING BY COFACTOR REDESIGN Introduction Natural Cofactors: Types, Occurrence, and Chemistry Inorganic Cofactors Organic Cofactors Redox Cofactors Concluding Remarks BIOCATALYST IDENTIFICATION BY ANAEROBIC HIGH-THROUGHPUT SCREENING OF ENZYME LIBRARIES AND ANAEROBIC MICROORGANISMS Introduction Oxygen-Sensitive Biocatalysts Biocatalytic Potential of Oxygen-Sensitive Enzymes and Microorganisms Anaerobic High-Throughput Screening Conclusions and Outlook ORGANOMETALLIC CHEMISTRY IN PROTEIN SCAFFOLDS Introduction Protocol/Practical Considerations Goals Summary ENGINEERING PROTEASE SPECIFICITY Introduction Protocol and Practical Considerations Concepts, Challenges, and Visions on Future Developments POLYMERASE ENGINEERING: FROM PCR AND SEQUENCING TO SYNTHETIC BIOLOGY Introduction PCR Sequencing Polymerase Engineering StrategiesSynthetic

Stefan Lutz holds a B. S. degree from the Zurich University of Applied Sciences (Switzerland), and a M.S. degree from the University of Teesside (UK). He then obtained a Ph.D. from the University of Florida and spent three years as a Postdoc with Stephen Benkovic at Pennsylvania State University under a fellowship of the Swiss National Science Foundation. Since 2002 he has been a Chemistry professor at Emory University in Atlanta, Georgia (USA). The research in the Lutz laboratory focuses on the structure-function relationship of proteins through combinatorial protein engineering.

Uwe Bornscheuer studied Chemistry at the University of Hannover (Germany), where he obtained a Ph. D. at the Institute of Technical Chemistry. He then spent a postdoctoral year at the University of Nagoya, Japan, before returning to Germany to join the Institute of Technical Biochemistry at the University of Stuttgart. Since 1999 he has been Professor for Biotechnology and Enzyme Catalysis at the University of Greifswald. His main research interest is the application of engineered enzymes in the synthesis of optically active compounds and in lipid modification.