Protein Targeting Protocols
(Sprache: Englisch)
In this updated volume, experts from around the world provide the latest protocols for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Emphasis is placed...
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In this updated volume, experts from around the world provide the latest protocols for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Emphasis is placed on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems. The book includes targeting protocols from different systems.
Inhaltsverzeichnis zu „Protein Targeting Protocols “
- Contents- Preface
- Contributors
- Bacterial systems
1. Localisation and mobility of bacterial proteins by confocal microscopy and fluorescence recovery after photobleaching (FRAP)
- Conrad Mullineaux
2. Membrane protein insertion and secretion in bacteria
- Jeanine de Keyzer, Martin van der Laan and Arnold J.M. Driessen
3. Identification of autotransporter proteins secreted by type V secretion systems in Gram-negative bacteria
- Yihfen T. Yen and Christos Stathopoulos
4. Periplasmic targeting of recombinant proteins in Escherichia coli
- Filipe J. M. Mergulhão and Gabriel A. Monteiro
5. In vitro analysis of the bacterial twin-arginine-dependent protein export
- Michael Moser, Sascha Panahandeh, Eva Holzapfel and Matthias Müller
- Eukaryotic systems
6. Nuclear import properties of the sex determining factor SRY
- Jade K. Forwood, Gurpreet Kaur and David A. Jans
7. The mitochondrial machinery for import of precursor protein, a review.
- Kip Gabriel and Nikolaus Pfanner
8. A genetic method to identify mitochondrial proteins in living mammalian cells
- Takeaki Ozawa and Yoshio Umezawa
9. In vitro and in vivo methods to study protein import into plant mitochondria
- Shashi Bhushan, Pavel F. Pavlov, Charlotta Rudhe and Elzbieta Glaser
10. Protein targeting to mitochondria of Saccharomyces cerevisiae and Neurospora crassa; in vitro and in vivo studies
- Panagiotis Paptheodorou, Gra_nya Doma_ska and Joachim Rassow
11. A mitosome purification protocol based on Percoll density gradients and its use in validating the mitosomal nature of Entamoeba histolytica mitochondrial Hsp70
- Jorge Tovar, Siân S.E. Cox and Mark van der Giezen
12. The chloroplast protein import machinery, a review
- Friederike Hörmann, Jürgen Soll and Bettina Bölter
13. Import of plastid precursor proteins into pea chloroplasts
- Jürgen Soll and Bettina Bölter
14. Protein targeting in "secondary" or "complex" chloroplasts
- Balbir K. Chaal and Beverley R. Green
15.
... mehr
Protein trafficking to the complex chloroplasts of Euglena
- Rostislav Vacula, Silvia Sláviková and Steven D. Schwartzbach
16. Fluorescent protein fusions for protein localization in plants
- John Runions, Chris Hawes and Smita Kurup
17. Genetic transformation; a tool to study protein targeting in diatoms
- Peter Kroth
18. Identification of proteins targeted into the endoplasmic reticulum by cDNA library screening
- Takeaki Ozawa and Yoshio Umezawa
19. Trafficking through the early secretory pathway of mammalian cells
- Theresa H. Ward
20. Studying protein export from the endoplasmic reticulum in plants
- Sally L. Hanton, Loren A. Matheson and Federica Brandizzi
21. Imaging the Golgi apparatus in living mitotic cells
- Nihal Altan-Bonnet
22. Evaluating yeast biosynthetic vacuolar transport
- Brian A. Davies, Darren S. carney and Bruce F. Horazdovsky
23. Targeting to lysosomes in mammalian cells: the biosynthetic and endocytic pathways
- Ann H. Erickson and Jeffrey P. Bocock
24. Monitoring autophagy in yeast: the Pho8_60 assay
- Daniel J. Klionsky
25. Protein targeting to yeast peroxisomes
- Ida van der Klei and Marten Veenhuis
26. Expression, localization and topology of fluorescently tagged plasma membrane-targeted transmembrane proteins
- Jeanne Shepshelovich and Koty Hirschberg
- General methods
27. Intracellular protein localization by immunoelectron microscopy
- Tetsuaki Osafune and Steven D. Schwartzbach
28. Separation of proteins by blue native electrophoresis (BN-PAGE)
- Olga Randelj, Joachim Rassow and Christian Motz
29. Computational prediction of subcellular localization
- Kenta Nakai and Paul Horton
30. Phylogenetic analysis to uncover organellar origins of nuclear-encoded genes
- Bernardo Foth
- Rostislav Vacula, Silvia Sláviková and Steven D. Schwartzbach
16. Fluorescent protein fusions for protein localization in plants
- John Runions, Chris Hawes and Smita Kurup
17. Genetic transformation; a tool to study protein targeting in diatoms
- Peter Kroth
18. Identification of proteins targeted into the endoplasmic reticulum by cDNA library screening
- Takeaki Ozawa and Yoshio Umezawa
19. Trafficking through the early secretory pathway of mammalian cells
- Theresa H. Ward
20. Studying protein export from the endoplasmic reticulum in plants
- Sally L. Hanton, Loren A. Matheson and Federica Brandizzi
21. Imaging the Golgi apparatus in living mitotic cells
- Nihal Altan-Bonnet
22. Evaluating yeast biosynthetic vacuolar transport
- Brian A. Davies, Darren S. carney and Bruce F. Horazdovsky
23. Targeting to lysosomes in mammalian cells: the biosynthetic and endocytic pathways
- Ann H. Erickson and Jeffrey P. Bocock
24. Monitoring autophagy in yeast: the Pho8_60 assay
- Daniel J. Klionsky
25. Protein targeting to yeast peroxisomes
- Ida van der Klei and Marten Veenhuis
26. Expression, localization and topology of fluorescently tagged plasma membrane-targeted transmembrane proteins
- Jeanne Shepshelovich and Koty Hirschberg
- General methods
27. Intracellular protein localization by immunoelectron microscopy
- Tetsuaki Osafune and Steven D. Schwartzbach
28. Separation of proteins by blue native electrophoresis (BN-PAGE)
- Olga Randelj, Joachim Rassow and Christian Motz
29. Computational prediction of subcellular localization
- Kenta Nakai and Paul Horton
30. Phylogenetic analysis to uncover organellar origins of nuclear-encoded genes
- Bernardo Foth
... weniger
Bibliographische Angaben
- 2nd ed. 2007, 520 Seiten, Maße: 16 x 24,1 cm, Gebunden, Englisch
- Herausgegeben: Mark van der Giezen
- Verlag: Humana Press
- ISBN-10: 1588297020
- ISBN-13: 9781588297020
- Erscheinungsdatum: 21.05.2007
Sprache:
Englisch
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