Signal Transduction Protocols

Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling.- Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes.- Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis.- RGS-insensitive Ga Subunits: Probes of Ga Subtype-selective Signaling and Physiological Functions of RGS Proteins.- Bioinformatic Approaches to Metabolic Pathways Analysis.- Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET.- Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes.- Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL).- Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization.- Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization.- Analysis of GPCR/Ion Channel Interactions.- Multicolor BiFC Analysis of G Protein bg Complex Formation and Localization.- Real-Time BRET Assays to Measure G Protein/Effector Interactions.- Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP.- Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters.- Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity.- Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells.- Visualizing Receptor Endocytosis and Trafficking.- Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM.- Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy.- Detection and Characterization of Receptor Interactions with PDZ Domains.- Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins.- Study of G Protein-Coupled Receptor/ßarrestinInteractions within

From the reviews of the third edition:"This well-illustrated book on signal transduction protocols is a valuable resource for scientists interested in learning the intricate details of complex signaling events that take place within the cell ... . Although written for the scientists in the field of signal transduction research, it also will be useful for graduate students and other investigators in pharmacology interested in this area. ... Given the rapid advances in the field of signal transduction, this third edition is essential." (Omer Iqbal, Doody's Book Reviews, March, 2012)