Stem Cell Culture

Subpopulations

VI Feeder Cells

VII Extracellular Matrix

VIII Culture Media

IX Monolayer Cultures of Cells

X Three�Dimensional Systems

Acknowledgments

Appendix 1: Nomenclature, Glossary, and Abbreviations

Appendix 2: Details on the Preparation of Buffers/Reagents

Appendix 3: Commercial Sources of Reagents

Appendix 4: Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells. (See Below for Suppliers and the Location of the Suppliers)

Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo

I Introduction

II Rationale

III Methods

IV Materials

V Discussion

VI Summary

Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells

I Introduction

II Rationale

III Methods

IV Materials

V Discussion

VI Summary

Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue

I Introduction

II Isolation of hMSCs from Trabecular Bone

III Isolation of hMSCs from Adipose Tissue

IV Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs

V Surface Marker Characterization of hMSCs

VI hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways

VII Summary

Culture of Mesenchymal Stem/Progenitor Cells in Adhesion�Independent Conditions

I Introduction

II Suspension Culture of Bone Marrow�Derived MPCs

III Analysis of Suspension Culture

IV Mechanism of MPC Expansion in Suspension

V Discussion

Purification and Long�Term Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes

I Introduction

II Materials and Methods: Cell Analysis and Purification by Flow Cytometry

III Materials and Methods: Long�Term Culture of Human Blood Vessel�Associated Progenitor Cells

IV Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cell