Stress Responses

Methods for Studying ER Stress and UPR Markers in Human Cells.- Assays for Induction of the Unfolded Protein Response and Selective Activation of the Three Major Pathways.- Assays to Characterize Molecular Chaperone Functions in vitro.- Analysis of Heat Shock Factor Complex in Mammalian HSP70 Promoter.- Immunofluorescence-Based Methods to Monitor DNA End Resection.- Visualizing the Spatiotemporal Dynamics of DNA Damage in Budding Yeast.- Detecting Reactive Oxygen Species by Immunohistochemistry.- Investigating Inflammasome Activation Under Conditions of Cellular Stress and Injury.- Methods for Studying microRNA Functions During Stress.- Measuring Autophagy in Stressed Cells.- Detection of Apoptosis Using Fluorescent Probes.- Measuring Death of Pancreatic Beta Cells in Response to Stress and Cytotoxic T Cells.- Adaptation of the Secretory Pathway in Cancer Through IRE1 Signaling.- Studying Nitrosative Stress in Parkinson's Disease.- Crosstalk Between ER Stress, Oxidative Stress, and Inflammation in Health and Disease.- Stress Responses During Ageing: Molecular Pathways Regulating Protein Homeostasis.- Targeting Stress Responses for Regenerative Medicine.

Christine M. Oslowski, Ph.D. 21 Franklin Street, Apt 7 Somerville, MA 02145 Cell: (617) 501-6711 Email: coslowsk@bu.edu