Toll-Like Receptors: Methods and Protocols

Part I: Methods to Detect and Analyze Toll-Like Receptors 1 Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells Jakub Siednienko and Sinead M. Miggin 2 Ligands, Cell-Based Models and Readouts Required for Toll-Like Receptor Action Jerome Dellacasagrande 3 Toll-Like Receptor Interactions Imaged by FRET Microscopy and GFP Fragment Reconstitution Gabor Horvath, Scott Young, and Eicke Latz 4 Predicting Toll-Like Receptor Structures and Characterizing Ligand Binding Joshua N. Leonard, Jessica K. Bell, and David M. Segal 5 Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures Tom P. Monie, Nicholas J. Gay, and Monique Gangloff 6 Expression, Purification and Crystallization of Toll/Interleukin-1 Receptor (TIR) Domains Xiao Tao and Liang Tong Part II: Methods to Analyze Signal Transduction Downstream of Toll-Like Receptor Stimulation 7 Proteomic Analysis of Protein Complexes in Toll-Like Receptor Biology Kiva Brennan and Caroline A. Jefferies 8 2D-DIGE Comparative Proteomics: Investigation of the Proteome in Cellular Signalling Pathways Nadia Ben Larbi and Caroline A. Jefferies 9 MAPPIT (Mammalian Protein-Protein Interaction Trap) Analysis of Early Steps in Toll-Like Receptor Signalling Peter Ulrichts, Irma Lemmens, Delphine Lavens, Rudi Beyaert, and Jan Tavernier 10 Analysis of the Functional Role of Toll-Like Receptor-4 Tyrosine Phosphorylation Andrei E. Medvedev and Wenji Piao 11 Analysis of Ubiquitin Degradation and Phosphorylation of Proteins Pearl Gray 12 The Generation of Highly Purified Primary Human Neutrophils and Assessment of Apoptosis in Response to Toll-Like Receptor Ligands Lisa C. Parker, Lynne R. Prince, David J. Buttle, and Ian Sabroe 13 Cellular Expression of A20 and ABIN-3 in Response to Toll-Like Receptor-4 Stimulation Kelly Vershelst, Lynn Verstrepen, Beatrice Coornaert, Isabelle Carpentier, and Rudi Beyaert 14 Characterisation of Viral Proteins that Inhibit Toll-Like Receptor Signal