Wildlife DNA Analysis
Applications in Forensic Science
(Sprache: Englisch)
DNA typing of non-human DNA is a fast developing area of research and professional practice. The application of DNA typing in wildlife forensic science is one of these prime uses of DNA typing and is gaining increasing profile. The use of DNA profiling in...
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DNA typing of non-human DNA is a fast developing area of research and professional practice. The application of DNA typing in wildlife forensic science is one of these prime uses of DNA typing and is gaining increasing profile. The use of DNA profiling in wildlife forensic science falls into two broad areas: species testing and genetic linkage. Species testing answers the question 'what species is this?' and genetic linkage answers the question 'did these two samples come from the same organism or population?' Wildlife DNA Analysis: Applications in Forensic Science provides an accessible introduction to both of these key areas. Clearly structured throughout, the introduction highlights the different types of crime where these techniques are regularly used. This chapter includes a discussion as to who performs forensic wildlife examinations, the standardisation and validation of methods, and the role of the expert witness in this type of alleged crime. This is followed by a detailed section on the science behind DNA typing including the problems in isolating DNA from trace material and subsequent genetic analysis are also covered. The book then undertakes a comprehensive review of species testing using DNA, including a step-by-step guide to sequence comparisons. A comparison of the different markers used in species testing highlights the criteria for a genetic marker. A full set of case histories illustrates the use of the different markers used. The book details the use of genetic markers to link two or more hairs/feather/leaves/needles to the same individual organism and the software used in population assignment. The problems and possibilities in isolating markers, along with the construction of allele databases are discussed in this chapter. The book concludes with evaluation and reporting of genetic evidence in wildlife forensic science illustrated by examples of witness statements. *An accessible introduction to this fast developing area of research within
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forensic science *Case studies throughout to link theory and practice and to highlight the use of DNA testing in species testing. *Covers both crimes against wildlife and offences where wildlife can provide vital evidence *Assumes only a basic background knowledge of DNA *Includes a comprehensive review of species testing using DNA, including a step-by-step guide to sequence comparisons
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Inhaltsverzeichnis zu „Wildlife DNA Analysis “
Foreword ixPreface xi
About the Authors xiii
Acknowledgements xv
1 Introduction 1
1.1 Importance of wildlife forensic science investigations 1
1.2 Role of forensic science in wildlife crimes 3
1.3 Legislation covering wildlife crime 4
1.4 Role of non-human DNA in forensic science 8
1.5 Development of wildlife DNA testing 9
1.5.1 History and current state of wildlife DNA forensic science 10
1.5.2 Wildlife forensic science testing 11
1.5.3 Performing DNA typing in wildlife investigations 13
1.6 Accreditation and certification 14
1.7 Standardisation and validation 20
1.8 Collection of evidential material, continuity of evidence and transportation
to the laboratory 24
1.9 Note taking and maintenance of a casefile 29
1.10 Case assessment and initial testing 30
1.11 Scope of book 32
Useful websites 32
References 33
2 DNA, Genomes and Genetic Variation 37
2.1 Introduction 37
2.2 The DNA molecule 37
2.3 Chromosomes and nuclear DNA 39
2.4 Genomes 41
2.4.1 Nuclear DNA 41
2.4.2 Mitochondrial and chloroplast DNA 44
2.5 DNA mutation and genetic variation 47
2.5.1 Genetic variation of repetitive DNA 48
2.5.2 Single base changes leading to genetic variation 48
2.5.3 Genetic loci used in species testing 50
2.6 DNA polymorphisms leading to speciation 53
2.6.1 Genetic isolation 54
2.6.2 Other processes leading to speciation 56
2.7 What is a species? 56
2.7.1 Subspecies 60
2.7.2 Genus to Kingdom 61
2.8 Summary 63
References 64
3 Methods in Wildlife Forensic DNA Analysis 69
3.1 Introduction 69
3.2 Protein polymorphisms 69
3.3 DNA isolation, purification and concentration 70
3.3.1 Generic
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aspects of DNA isolation 70
3.3.2 Lysis step 71
3.3.3 DNA purification: silica-based extraction 72
3.3.4 DNA purification: Chelex R 100 resin 73
3.3.5 DNA purification: organic extraction 74
3.3.6 Microconcentration 76
3.4 DNA quantification 76
3.5 Restriction fragment length polymorphisms (RFLP) 78
3.6 Methods based on the polymerase chain reaction 81
3.6.1 Factors affecting PCR efficiency and optimisation of PCR 84
3.6.2 PCR-based methods of DNA quantification 88
3.6.3 Random amplification of polymorphic DNA 91
3.6.4 Amplification of fragment length polymorphisms (AFLP) 93
3.7 PCR set-up 95
3.8 PCR clean-up 98
3.9 DNA sequencing 99
3.10 SNP typing 100
3.11 New generation of DNA sequence methods 102
Suggested reading 104
4 Species Testing 105
4.1 Introduction 105
4.2 Species 106
4.2.1 Genetic variation and correspondence with taxonomy 106
4.3 Attributes of a species testing locus 106
4.4 Application of a locus to a species 110
4.5 Tests available and how they are performed 110
4.5.1 Sequencing 111
4.5.2 Species-specific primers 124
4.6 Developing a species test 127
4.6.1 Use of data on GenBank and sequence alignment 128
4.6.2 Designing primers 135
4.6.3 Validation 156
4.7 Interpretation and reporting of results 159
4.7.1 Interpretation and reporting sequencing results 160
4.7.2 Interpretation and reporting species-specific testing results 169
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3.3.2 Lysis step 71
3.3.3 DNA purification: silica-based extraction 72
3.3.4 DNA purification: Chelex R 100 resin 73
3.3.5 DNA purification: organic extraction 74
3.3.6 Microconcentration 76
3.4 DNA quantification 76
3.5 Restriction fragment length polymorphisms (RFLP) 78
3.6 Methods based on the polymerase chain reaction 81
3.6.1 Factors affecting PCR efficiency and optimisation of PCR 84
3.6.2 PCR-based methods of DNA quantification 88
3.6.3 Random amplification of polymorphic DNA 91
3.6.4 Amplification of fragment length polymorphisms (AFLP) 93
3.7 PCR set-up 95
3.8 PCR clean-up 98
3.9 DNA sequencing 99
3.10 SNP typing 100
3.11 New generation of DNA sequence methods 102
Suggested reading 104
4 Species Testing 105
4.1 Introduction 105
4.2 Species 106
4.2.1 Genetic variation and correspondence with taxonomy 106
4.3 Attributes of a species testing locus 106
4.4 Application of a locus to a species 110
4.5 Tests available and how they are performed 110
4.5.1 Sequencing 111
4.5.2 Species-specific primers 124
4.6 Developing a species test 127
4.6.1 Use of data on GenBank and sequence alignment 128
4.6.2 Designing primers 135
4.6.3 Validation 156
4.7 Interpretation and reporting of results 159
4.7.1 Interpretation and reporting sequencing results 160
4.7.2 Interpretation and reporting species-specific testing results 169
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Autoren-Porträt von Adrian Linacre, Shanan Tobe
Adrian LinacreFlinders University, Adelaide, Australia
Shanan Tobe
University of Strathclyde, Glasgow, U
Bibliographische Angaben
- Autoren: Adrian Linacre , Shanan Tobe
- 2013, 2. Aufl., 350 Seiten, Maße: 16,7 x 24,4 cm, Kartoniert (TB), Englisch
- Verlag: Wiley & Sons
- ISBN-10: 0470665963
- ISBN-13: 9780470665961
- Erscheinungsdatum: 10.05.2013
Sprache:
Englisch
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