Yeast Protocols

1. Basic Investigations in Saccharomyces cerevisiae

            Brendan Curran and Virginia Bugeja

2. Isolation of Yeast Nucleic Acids

            Michael Biss, Michelle Hanna and Wei Xiao

3. Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry

            Ting Wei and Heping Dai

4. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

            R. Daniel Gietz

5. Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe

            Johannes H. Hegemann, Sven Boris Heick, Jennifer Pöhlmann, Markus Langen and Ursula Fleig

6. See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry

            Changhui Deng and Andrew N. Krutchinsky

7. Yeast Two Hybrid Liquid Screening

            Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz

8. Targeted Mutagenesis of a Specific Gene in Yeast

            Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz

9. Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae

            Kai Stefan Dimmer

10. Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy

            Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie

11. Assays for Autophagy II: Mitochondrial Autophagy

            Tomotake Kanki and Koji Okamoto

12. Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction

            Danielle Hagstrom and Changle Ma

13. Yeast Survival and Growth Assays

            Xin Xu, Amanda Lambrecht, and Wei Xiao

14. Spontaneous Mutagenesis Assay

            Susan Blackwell, Michelle Hanna, and Wei Xiao

15. Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene

            Bernard A. Kunz

16. piggyBac Transposon-Based Insertional Mutagenesis for the Fission