Resin Microscopy and On-Section Immunocytochemistry / Springer Lab Manuals (PDF)
(Sprache: Englisch)
Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. Resin-embedded...
sofort als Download lieferbar
Printausgabe 160.49 €
eBook (pdf) -7%
149.79 €
74 DeutschlandCard Punkte sammeln
- Lastschrift, Kreditkarte, Paypal, Rechnung
- Kostenloser tolino webreader
Produktdetails
Produktinformationen zu „Resin Microscopy and On-Section Immunocytochemistry / Springer Lab Manuals (PDF)“
Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired and appropriate choices made.
In the first part of the book, the background of the various resins available are discussed and information on inexpensive alternative technologies where they exist is provided. The various steps involved in tissue processing, beginning with fixation, are first described in theory, then detailed protocols are presented for their application, including troubleshooting sections. The second part rationalises the great variety of labelling methods that are commonly used for "on-section" cytochemistry and immunocytochemistry, where colloidal gold is currently overwhelmingly the most popular marker. The principles behind the usage of various markers and their limitations and advantages are discussed.
In the first part of the book, the background of the various resins available are discussed and information on inexpensive alternative technologies where they exist is provided. The various steps involved in tissue processing, beginning with fixation, are first described in theory, then detailed protocols are presented for their application, including troubleshooting sections. The second part rationalises the great variety of labelling methods that are commonly used for "on-section" cytochemistry and immunocytochemistry, where colloidal gold is currently overwhelmingly the most popular marker. The principles behind the usage of various markers and their limitations and advantages are discussed.
Bibliographische Angaben
- Autoren: Geoffrey R. Newman , Jan A. Hobot
- 2012, 2nd ed. 2001, 273 Seiten, Englisch
- Verlag: Springer Berlin Heidelberg
- ISBN-10: 3642569307
- ISBN-13: 9783642569302
- Erscheinungsdatum: 06.12.2012
Abhängig von Bildschirmgröße und eingestellter Schriftgröße kann die Seitenzahl auf Ihrem Lesegerät variieren.
eBook Informationen
- Dateiformat: PDF
- Größe: 26 MB
- Ohne Kopierschutz
- Vorlesefunktion
Sprache:
Englisch
Kommentar zu "Resin Microscopy and On-Section Immunocytochemistry / Springer Lab Manuals"
0 Gebrauchte Artikel zu „Resin Microscopy and On-Section Immunocytochemistry / Springer Lab Manuals“
Zustand | Preis | Porto | Zahlung | Verkäufer | Rating |
---|
Schreiben Sie einen Kommentar zu "Resin Microscopy and On-Section Immunocytochemistry / Springer Lab Manuals".
Kommentar verfassen