TGF-ß Signaling / Methods in Molecular Biology Bd.1344 (PDF)
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This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF-ß from the basic principles to the practical applications. Chapters provide basic introduction of TGF-ß signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-ß signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.
- 2015, 1st ed. 2016, 394 Seiten, Englisch
- Herausgegeben: Xin-Hua Feng, Pinglong Xu, Xia Lin
- Verlag: Springer, New York
- ISBN-10: 1493929666
- ISBN-13: 9781493929665
- Erscheinungsdatum: 31.10.2015
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- Größe: 12 MB
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