Basic Protocols in Foods and Nutrition

Experimental Diets (Normocaloric and Hypercaloric Diets).- Measures of Food Intake, Body Weight Gain, and Energy Efficiency in Mice.- The assessment of glucose homeostasis in rodents: Glucose, Insulin and Pyruvate.- Histopathological Evaluation of Steatohepatitis in Animal Experiments.- Quantification of short-chain fatty acids in feces.- Quantification of non-esterified fatty acids in serum and plasma.- Lipid Peroxidation (TBARS) in biological samples.- Extraction of Bile Acids from Biological Samples and Quantification Using Ultra-High-Performance Liquid Chromatography-Orbitrap Mass Spectrometry.- Glycogen measurement.- Evaluation of the minerals and trace elements in the biological samples.- Microbiome Evaluation.- The GSH colorimetric method as measurement of antioxidant status in serum and rodent tissues.- Guidance for designing a preclinical bioavailability study of bioactive compounds.- Protocols related to Nutritional Anamnesis in Head and Neck Cancer Patients.- Food Diary, Food Frequency Questionnaire, and 24-hour dietary recall.- The Use of Questionnaires to Measure Appetite.- Protocols for the Use of Indirect Calorimetry in Clinical Research.- Anthropometric Assessment Methods for Adults and Older People.- MRI-based Body Composition Analysis.- Functional tests for assessing human beta-cell function and insulin sensitivity.- Maximum oxygen consumption - VO2max laboratory assessment.- Muscle and Fat Biopsy and Metabolomics.