Human Cell Culture Protocols

Introduction to Cell Culture.- Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocytes.- Isolation, Cultivation, and Application of Human Alveolar Epithelial Cells.- Culture of Parathyroid Cells.- Functional Analysis of Human Islets of Langerhans Maintained in Culture.- Conversion of Non-Endocrine Human Pancreatic Cells to Insulin Producing Cells for Treatment of Diabetes.- Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro.- Human Hepatocytes: Isolation, Culture, and Quality Procedures.- Preclinical Testing of Virotherapeutics for Primary and Secondary Tumors of the Liver.- Isolation and Functional Studies of Human Fetal Gastric Epithelium in Primary Culture.- Isolation, Characterization, and Culture of Normal Human Intestinal Crypt and Villus Cells.- Primary Culture of Human Renal Proximal Tubule Epithelial Cells and Interstitial Fibroblasts.- Glomerular Epithelial and Mesangial Cell Culture and Characterization.- Isolation and Culture of Human Adipocytes and Adipose Tissue.- Primary Culture of Human Adipocyte Precursor Cells: Expansion and Differentiation.- Primary Culture of Ovarian Cells for Research on Cell Interactions in the Hormonal Control of Steroidogenesis.- Human Vascular Smooth Muscle Cell Culture.- Culture of Human Endothelial Cells from Umbilical Veins.- Human Peripheral Blood Mononuclear Cell Culture for Flowcytometric Analysis of Phosphorylated Mitogen-Activated Protein Kinases.- Human CD4+CD25highCD127low/neg Regulatory T Cells.- Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation.- Isolation and Culture of Human Osteoblasts.- Human Osteoclast Culture and PhenotypicCharacterization.- Effects of Temperature Generated from the Holmium:YAG Laser on Human Osteoblasts in Monolayer Tissue Culture.- Laser Microdissection Microscopy: Application to Cell Culture.- Automated Adherent Human Cell Culture (Mesenchymal Stem Cells).- Culturing and Differentiating Human