Integrin Protocols

Working with Integrins.- Integrin-Binding Peptides Derived from Phage Display Libraries.- Production of Rat Monoclonal Antibodies Specific for Mouse Integrins.- Biochemical Analysis of Integrin-Mediated Shc Signaling.- Assays for Kinase Activity.- Immunoblotting of Integrins.- Application of Flow Cytometry in the Analysis and Sterile Sorting of Cell Populations Based on Integrin Expression.- Expression of Integrin Transcripts in Human Cancer Cells.- Functional Analysis of Integrins.- Functional Analysis of the ?2 Integrins.- Expression of Heterologous Integrin Genes.- Preparing a Polyclonal Antibody to Mouse ?1 Integrin with Function-Blocking Activity.- Integrin Gene Targeting.- Determination of Positive and Negative Regulatory Elements.- Integrin Function at the Cellular Level.- A Cell-Based Adhesion Assay for the Characterization of Integrin ?v?3 Antagonists.- Integrins in Cell Migration.- Models for Studying Cellular Invasion of Basement Membranes.- Methods for Studying Anoikis.- Use of the 10-Day-Old Chick Embryo Model for Studying Angiogenesis.- Cell Shape and Integrin Signaling Regulate the Differentiation State of Mammary Epithelial Cells.

"Integrin Protocols aims to provide a hand-book of methods for the study of integrins. Many of the essential techniques are covered, with sections ranging from the relatively straight-forward (immunoblotting) to the highly complex (phage display and knock-outs)...Over half of the sections are by major figures in the integrin field, thus giving the book considerable authority..."-Cell Biology International 2000