Methods in Molecular Biology / Electron Microscopy
Methods and Protocols
(Sprache: Englisch)
This book presents the newest technology in electron microscopy. It comprises two major areas of electron microscopy - transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The volume provides clear, concise instructions on...
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Produktinformationen zu „Methods in Molecular Biology / Electron Microscopy “
This book presents the newest technology in electron microscopy. It comprises two major areas of electron microscopy - transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. A notes section enables efficient adaptation and troubleshooting of protocols.
Klappentext zu „Methods in Molecular Biology / Electron Microscopy “
In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Likethe first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.
Inhaltsverzeichnis zu „Methods in Molecular Biology / Electron Microscopy “
CONTENTSElectron Microscopy: Methods and Protocols (2nd Edition)
"METHODS IN MOLECULAR BIOLOGY" Volume 117
1. Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells
John J. Bozzola (USA)
2. Processing Biological Tissues for Ultrastructural Study
José A. Mascorro (USA) and John J. Bozzola (USA)
3. Processing Plant Tissues for Ultrastructural Study
John Kuo and Ivan Kuo (Australia)
4. Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy
Paul Webster (USA)
5. Ultramicrotomy: for Biological Electron Microscopy
Herbert K. Hagler (USA)
6. Post Staining Grids for Transmission Electron Microscopy: Conventional and Alternative Protocols
E. Ann Ellis (USA)
7. Negative Staining of Thinly Spread Biological Samples
J. Robin Harris (Germany)
8. Recent Advances in High Pressure Freezing: Equipment and Specimen Loading Methods
Kent L McDonald, Mary Morphew, Paul Verkade, and Thomas Müller-Reichert (USA/Germany)
9. Cryoultramicrotomy: Cryo-Electron Microscopy of Vitreous Sections
Dimitri Vanhecke, Luca Studer and Daniel Studer (Switzerland)
10. Cell-free Extract Systems and the Cytoskeleton: Preparation of Biochemical Experiments for Transmission Electron Microscopy
Margaret Coughlin, William M. Brieher and Ryoma Ohi (USA)
11. Electron Microscopy In Situ Hybridization - Tracking DNA and RNA Sequences at High Resolution
Du_an Cmarko and Karel Koberna (Chezvakea)
12. Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections
Heinz Schwarz and Bruno M. Humbel (Germany/Netherlands)
13. Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry
Paul Webster and Alexandre Webster (USA)
14. F-TEM mages: Integrating Fluorescence Microscopy with Transmission Electron Microscopy
Paul A. Sims and Jeff D. Hardin (USA)
15. Quantitative Immunoelectron Microscopy: Alternative Ways of Assessing Subcellular Patterns
of Gold Labelling
Terry M. Mayhew
... mehr
(England)
16. Electron Crystallography of Membrane Proteins
Hui-Ting Chou, James Evans and Henning Stahlberg (USA)
17. Cryo-Electron Microscopy of Icosahedral Virus Particle
Wen Jiang and Wah Chiu (USA)
18. 3D Reconstructions of Chromosomes with Electron Tomography
Peter Engelhardt (Finland)
19. Whole-Mount Immuno-Electron Tomography of Chromosomes and Cells
Peter Engelhardt, Jari Meriläinen, Fang Zhao, Susumu Uchiyama, Kiichi Fukui
and Veli-Pekka Lehto (Finland/Japan)
20. 3-D Cryo-Transmission Electron Microscopy of Cells and Organelles
Michael Marko and Chyong-Ere Hsieh (USA)
21. Electron Energy-Loss Spectroscopy (EELS) as a Tool for Elemental Analysis in Biological Specimens
Nadine Kapp, Peter Gehr, Daniel Studer and Marianne Geiser (Switzerland)
22. Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens
John J. Bozzola (USA)
23.Variable Pressure (VPSEM) and Environmental Scanning Electron Microscopy (ESEM): Imaging of
Biological Samples
Brendan J. Griffin (Australia)
24. Cryo-planing Technique for Visualizing the Distribution of Water in Woody Tissue by Cryo-Scanning Electron Microscopy
Yasuhiro Utsumi and Yuzou Sano (Japan)
25. X-ray Microanalysis in the Scanning Electron Microscope
Godfrield M Roomans and Anca Dragomir (Sweden)
26. Botanical SEM X-ray Microanalysis in Cryo-Scanning Electron Microscopy
Beat Frey (Switzerland)
27. Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research
Nicholas P. Lockyer (England)
28. Using SIMS and MIMS in Biological Materials: Application to Higher Plants
Nicole Grignon (France)
16. Electron Crystallography of Membrane Proteins
Hui-Ting Chou, James Evans and Henning Stahlberg (USA)
17. Cryo-Electron Microscopy of Icosahedral Virus Particle
Wen Jiang and Wah Chiu (USA)
18. 3D Reconstructions of Chromosomes with Electron Tomography
Peter Engelhardt (Finland)
19. Whole-Mount Immuno-Electron Tomography of Chromosomes and Cells
Peter Engelhardt, Jari Meriläinen, Fang Zhao, Susumu Uchiyama, Kiichi Fukui
and Veli-Pekka Lehto (Finland/Japan)
20. 3-D Cryo-Transmission Electron Microscopy of Cells and Organelles
Michael Marko and Chyong-Ere Hsieh (USA)
21. Electron Energy-Loss Spectroscopy (EELS) as a Tool for Elemental Analysis in Biological Specimens
Nadine Kapp, Peter Gehr, Daniel Studer and Marianne Geiser (Switzerland)
22. Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens
John J. Bozzola (USA)
23.Variable Pressure (VPSEM) and Environmental Scanning Electron Microscopy (ESEM): Imaging of
Biological Samples
Brendan J. Griffin (Australia)
24. Cryo-planing Technique for Visualizing the Distribution of Water in Woody Tissue by Cryo-Scanning Electron Microscopy
Yasuhiro Utsumi and Yuzou Sano (Japan)
25. X-ray Microanalysis in the Scanning Electron Microscope
Godfrield M Roomans and Anca Dragomir (Sweden)
26. Botanical SEM X-ray Microanalysis in Cryo-Scanning Electron Microscopy
Beat Frey (Switzerland)
27. Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research
Nicholas P. Lockyer (England)
28. Using SIMS and MIMS in Biological Materials: Application to Higher Plants
Nicole Grignon (France)
... weniger
Bibliographische Angaben
- Autor: John Kuo
- 2nd ed., 608 Seiten, 5 farbige Abbildungen, 209 Schwarz-Weiß-Abbildungen, Maße: 16,5 x 23,3 cm, Gebunden, Englisch
- Herausgegeben: John Kuo
- Verlag: Springer, Berlin
- ISBN-10: 1588295737
- ISBN-13: 9781588295736
- Erscheinungsdatum: 02.05.2007
Sprache:
Englisch
Rezension zu „Methods in Molecular Biology / Electron Microscopy “
"This is an excellent book, with well-written chapters. The protocols are easy to follow and well organized. This book is highly recommended for beginners to advanced level microscopists." -Micron
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