Mitosis
Methods and Protocols
(Sprache: Englisch)
With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods...
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With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.
Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.
Klappentext zu „Mitosis “
With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.
Inhaltsverzeichnis zu „Mitosis “
1. Knock-In and Knock-Out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways- Alexis R. Barr, Deborah Zyss, and Fanni Gergely
2. Functional Dissection of Mitotic Regulators through Gene Targeting in Human Somatic Cells
- Eli Berdougo, Marie-Emilie Terret, and Prasad V. Jallepalli
3. RNAi in Drosophila S2 Cells As A Tool For Studying Cell Cycle Progression
- Mónica Bettencourt-Dias and Gohta Goshima
4. Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast
- Marco Geymonat, Adonis Spanos, and Steven Sedgwick
5. Hydrodynamic Analysis of Human Kinetochore Complexes during Mitosis
- Sarah E. McClelland and Andrew D. McAinsh
6. Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells
- Pier Paolo D'Avino, Vincent Archambault, Marcin R. Przewloka, Wei Zhang, Ernest D. Laue, and David M. Glover
7. Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines
- Michael H. A. Schmitz and Daniel W. Gerlich
8. Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos
- Eileen O'Toole and Thomas Müller-Reichert
9. Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells
- António J. Pereira, Irina Matos, Mariana Lince-Faria, and Helder Maiato
10. Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells
- Suzanna L. Prosser and Andrew M. Fry
11. Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin under the Native Promoter
- Masamitsu Sato, Mika Toya, and Takashi Toda
12. Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression
- Christiane Klebig, Alberto Toso, Satyarebala Borusu, and Patrick Meraldi
13. Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
- Michael J. Emanuele and P. Todd Stukenberg
14. Live Cell
... mehr
Imaging of Kinetochore Capture by Microtubules in Budding Yeast
- Kozo Tanaka and Tomoyuki U. Tanaka
15. The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery
- Josefin Fernius and Kevin G. Hardwick
16. Measuring Proteolysis in Mitosis
- Catherine Lindon and Barbara Di Fiore
17. An In Vitro Assay for Cdc20-dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast
- Scott C. Schuyler and Andrew W. Murray
18. In vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg Extracts
- Hiroyuki Yamano, Michelle Trickey, Margaret Grimaldi, and Yuu Kimata
19. Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation
- Adam Williamson, Lingyan Jin, and Michael Rape
20. Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive ("Pseudosubstrate") Inhibitors
- Matthew K. Summers and Peter K. Jackson
- Kozo Tanaka and Tomoyuki U. Tanaka
15. The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery
- Josefin Fernius and Kevin G. Hardwick
16. Measuring Proteolysis in Mitosis
- Catherine Lindon and Barbara Di Fiore
17. An In Vitro Assay for Cdc20-dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast
- Scott C. Schuyler and Andrew W. Murray
18. In vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg Extracts
- Hiroyuki Yamano, Michelle Trickey, Margaret Grimaldi, and Yuu Kimata
19. Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation
- Adam Williamson, Lingyan Jin, and Michael Rape
20. Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive ("Pseudosubstrate") Inhibitors
- Matthew K. Summers and Peter K. Jackson
... weniger
Bibliographische Angaben
- 2009, XI, 345 Seiten, Maße: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben: Andrew D. McAinsh
- Verlag: Springer, Berlin
- ISBN-10: 160327992X
- ISBN-13: 9781603279925
- Erscheinungsdatum: 29.06.2009
Sprache:
Englisch
Rezension zu „Mitosis “
From the reviews:"McAinsh ... introduces mitosis as a phenomenally complicated process of the splitting of genetic material into two daughter cells that prevents genomic instability. Twenty chapters comprise this collection of laboratory protocols of up-to-date approaches and methods ... used to investigate the mechanism of mitosis at the molecular level. ... The volume is intended for graduate students and researchers in the field." (SciTech Book News, March, 2010)
Pressezitat
From the reviews: "McAinsh ... introduces mitosis as a phenomenally complicated process of the splitting of genetic material into two daughter cells that prevents genomic instability. Twenty chapters comprise this collection of laboratory protocols of up-to-date approaches and methods ... used to investigate the mechanism of mitosis at the molecular level. ... The volume is intended for graduate students and researchers in the field." (SciTech Book News, March, 2010)
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