Nitric Oxide Research from Chemistry to Biology: EPR Spectroscopy of Nitrosylated Compounds

Nitrification and Nitrogen-Fixation.- Bacterial Nitric Oxide Synthase.- Discussion and Conclusion.- 8. Nitric Oxide Biosynthesis in Mammals.- Constitutive and Inducible Nitric Oxide Synthases.- Enzymatic Mechanism of Nitric Oxide Synthases.- Nitric Oxide Synthases' Regulations.- Post-Transcriptional Modifications of the Enzyme.- Conclusion.- 9. The Use of EPR Spectroscopy for the Identification of the Nature of Endothelium-Derived Relaxing Factor.- The Nature of Endothelium-Derived Relaxing Factor: A Controversy.- Nitric Oxide as an EDRF Detected by EPR Spectroscopy.- Fe(SR)2(NO)2 Ternary Iron Complexes as Plausible EDRF or Nitric Oxide Transporters.- Conclusion.- 10. Enzymatic Targets of Nitric Oxide as Detected by EPR Spectroscopy Within Mammal Cells.- Soluble Guanylate Cyclase.- [FeS] Cluster-Containing Enzyme.- Ribonucleotide Reductase.- Proteins of the Iron Metabolism.- Conclusion.- 11. Overproduction of Nitric Oxide in Physiology and Pathophysiology: EPR Detection.- Cancer and Interleukin-2 Immunotherapy.- Parasites, Fungi and Bacterial Infections.- Septic Shock.- Autoimmune Diseases.- Organ Transplantations.- Vascular Diseases.- Other Physiological and Pathophysiological States.- Conclusion.- 12. Palliatives to Underproduction of Nitric Oxide as Assayed by EPR Spectroscopy.- Metal Complexes as Nitric Oxide Donors.- Nitrite, Organic Nitrites and Nitrates.- Other Derivatives.- Inhalation Of Nitric Oxide: NO Gas, a Suppletive Drug.- Conclusion.- 13. Nitric Oxide-Specific Spin-Trapping EPR Methods.- Use of Hemoglobin as a Nitric Oxide Spin-Trap.-Conventional Spin-Trapping.- Prospects on Nitric Oxide-Specific Spin-Traps.- Scavenging of Nitric Oxide by Reaction with Stable Free Radicals.- Line Broadening of Fusinite and Other Free Radicals by Nitric Oxide.- Use of Fe-(DETC)2 and Similar Complexes as Nitric Oxide Spin-Traps.- Conclusion.- 14. General Discussion: Crossregulations of Metalloenzymes Triggered by Nitric Oxide.- Downregulation of Nitric Oxide Synthase by Nitric Oxide.- Inhibition of Microsomal Cytochromes P-450.- The Urea Cycle.- Inhibition or Regulation of Cytochrome c Oxidase.- Crossregulations of Other Inducible Metalloproteins.- Regulation by Nitric Oxide of Other Metalloenzymes of the Inflammatory Response.- Other Targets.- General Conclusion.