Proteomics in Practice
A Guide to Successful Experimental Design. Includes a detailed description of the DIGE technology
(Sprache: Englisch)
Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.
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Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.
Klappentext zu „Proteomics in Practice “
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Inhaltsverzeichnis zu „Proteomics in Practice “
- Preface- Foreword
- Abbreviations
- Glossary of terms
- INTRODUCTION
- History
- Critical points
- Hypothesis driven approach vs. High Throughput
- Complementarities of workflows
- Concept of experimental planning
- Success stories, highlights of useful references
PART I: PROTEOMICS TECHNOLOGY
- Electrophoresis
- Liquid Chromatography
- Mass spectrometry
- Bioinformatics tools / data mining
- Methods under development
PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS
- Strategy where to start
- Gel-based workflow
- LC-based workflow
- Latest trends
- Validation with Western blotting
- Statistic evaluation
PART III: TROUBLE SHOOTING
- Gel-based workflow
- LC based workflow
- References
- Index
Autoren-Porträt von Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Reiner Westermeier gained his doctorate in 1981 at the Technical University of Munich, Germany. There he remained engaged in the development of new electrophoresis systems and applications until 1983, a period that also involved several international collaborations and lecturing tours.From 1984 to 1987 Reiner Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese-Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech, later GE Healthcare since July 1997, and its Senior Scientist for Proteomics since January 2002.
Reiner Westermeier is the author of several publications and two books, both published by WILEY-VCH.
Tom Naven and Hans-Rudolf Höpker are also working for GE Healthcare as proteomics scientists.
Bibliographische Angaben
- Autoren: Reiner Westermeier , Tom Naven , Hans-Rudolf Höpker
- 2008, 2. Aufl., XX, 482 Seiten, 28 farbige Abbildungen, 131 Schwarz-Weiß-Abbildungen, Maße: 17,8 x 24,6 cm, Gebunden, Englisch
- Verlag: Wiley-VCH
- ISBN-10: 3527319417
- ISBN-13: 9783527319411
- Erscheinungsdatum: 26.03.2008
Sprache:
Englisch
Rezension zu „Proteomics in Practice “
"This book will be a valuable addition to any laboratory engaged in proteomics and is highly recommended." ( Internet Journal of Genomics and Proteomics 2008)
Pressezitat
"This book will be a valuable addition to any laboratory engaged in proteomics and is highly recommended." ( Internet Journal of Genomics and Proteomics 2008)
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